Rhizoglomus intraradices Improves Plant Growth, Root Morphology and Phytohormone Balance of Robinia pseudoacacia in Arsenic-Contaminated Soils

• Main Authors: QiaoMing Zhang, Minggui Gong, Kaiyang Liu, Yanlan Chen, Jiangfeng Yuan, Qingshan Chang
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2020-07-01
• Series: Frontiers in Microbiology
• Subjects: arbuscular mycorrhizal fungi; Rhizoglomus intraradices; Robinia pseudoacacia; endogenous phytohormone; root morphology; arsenic stress
• Online Access: https://www.frontiersin.org/article/10.3389/fmicb.2020.01428/full

Arbuscular mycorrhizal fungi (AMF) are known to improve the resistance of host plants against various heavy metal stresses. However, the arsenic (As) resistance mechanism of AMF-inoculated woody legumes remains unclear. In this study, black locust (Robinia pseudoacacia L.) seedlings were cultivated in potted soils inoculated with or without AMF Rhizoglomus intraradices under three different levels of As stress (0, 100, and 200 mg As kg–1 soil) over 4 months. The objective of this paper was to investigate the effects of AMF on plant growth, root morphology, and the content and ratio of endogenous phytohormones and soil glomalin under As stress condition. As stress toxicity suppressed the AM spore germination and colonization, plant growth, and the content of soil glomalin and changed the morphological characteristics of the roots and the balance of endogenous hormone levels in plants. However, R. intraradices inoculation improved the shoot and root dry weights, total root length, root surface area, root volume, and the number of root forks and tips across all As treatments. R. intraradices inoculation obviously decreased the percentage of root length in the 0- to 0.2-mm diameter class and increased those in the 0.5- to 1.0-mm and >1.0-mm diameter classes; the percentages in the 0.2- to 0.5-mm diameter class were less affected by R. intraradices inoculation. The concentrations of the easily extractable glomalin-related (EE-GRSP) and total glomalin-related soil protein (T-GRSP) were higher in the of R. intraradices-inoculated seedlings than those in the non-inoculated seedlings. Furthermore, R. intraradices inoculation increased the concentrations of indole-3-acetic acid (IAA) and abscisic acid (ABA), but decreased the concentrations of gibberellic acid (GA) and zeatin riboside (ZR). The phytohormone ratios of IAA/ABA, GA/ABA, ZR/ABA, and (IAA + GA + ZR)/IAA in the R. intraradices-inoculated seedlings were lower than those in the non-inoculated seedlings. These results indicated that R. intraradices alleviated As toxicity in R. pseudoacacia seedlings by improving their plant growth, altering root morphology, regulating the concentrations and ratios of phytohormones, and increasing the concentration of soil glomalin. The results suggested that AMF-inoculated R. pseudoacacia seedlings would be a critical factor in successful vegetation restoration and soil development in As-contaminated soils.