Validação de método para determinação de ácidos orgânicos voláteis em efluentes de reatores anaeróbios empregando cromatografia líquida

• Main Authors: Maristela Barnes Rodrigues Cerqueira, Adriana Neves Dias, Sergiane Souza Caldas, Fabrício Butierres Santana, Marcelo Gonçalves Montes D'Oca, Ednei Gilberto Primel
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2011-01-01
• Series: Química Nova
• Subjects: VFAs; matrix effect; HPLC-DAD
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000100029&lng=en&tlng=en
• ISSN: 1678-7064

This work deals with the method validation for the determination of acetic, propionic and butyric acids (VFAs) in wastewaters from anaerobic reactors by HPLC-DAD. Separation was performed using a C18 column and the mobile phase composition were water pH 3.0 and methanol 90:10 (v/v). The detection and quantification was carried out at 220 nm. The method shows good linearity (r²>0.996), with adequate accuracy (89-102%) and relative standard deviations lower than 18%. The matrix effect was considered low (-4.1, -3.9 and 1.4%). The developed method is fast, simple and cheap; and it was applied in wastewater samples from anaerobic reactor.