The ability inhibition Gumboro disease virus in 3-week-old chickens of chicken Interferon Alpha

• Main Authors: Nguyễn Thị Thanh Giang, Hồ Quảng Đồ, Nguyễn Đăng Quân
• Format: Article
• Language: English
• Published: HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE 2020-11-01
• Series: Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
• Subjects: interferon; gumboro; gà; rchifn-α
• Online Access: https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/1017
• ISSN: 2734-9322; 2734-9594

Virus-infectious diseases are permanent threats and cause serious lost for chicken industry of our country. Recombinant chicken interferon alpha (rChIFN-α) were expressed successfully on yeast pichia pastorisand was functional antivirus. The objective of this study was determined recombinant chicken interferon alpha (rChIFN-α) effectiveness in 3-week-old chickens infected infectious bursal diseasevirus (IBDV).In the present study, we used 3-week-old chickens without IBDV antibodies. Each 3-week-old chickens were infected virulent Gumboro disease virus with dose of 5x104ELD50. After 24 hours infected virus, they were exposed to eye/nasal drops with one of doses 0.1 μg/chick; 1 μg/chick; 10 μg/chick or 100 μg/chick. The results suggested treatment with rChIFN-α 0.1 μg/chick, the chickens' protected and survival rate were 46.7% and 80% respectively. Group of chickens were treated rChIFN-α 1 μg / chick, the protected and survival rate of chickens respectively 60.0% and 86.7%; The same result with chickens were treated rChIFN-α 10 μg/chick and 100 μg/chick, the chickens' protected and survival rate were 66.7% and 93.3%, respectively. Meanwhile, the positive control group (chicken infected virus, untreated), unprotected chickens (infection rate was 100%) and survival rate was only 60,00%. The negative control (chickens uninfected viruses, untreated with rChIFN-α), completely uninfected and 100% survival chickens. The result suggested that rChIFN-α have effective treating Gumboro disease and depends on the dose used. In addition, the chickens' antibody titres shown that the chickens' immune response in rChIFN-α groups was not different than the chickens infected only Gumboro disease virus, suggested that rChIFN-α did not affect the chickens' immune response.