Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses
An indole−3−acetic acid producing <i>Bacillus altitudinis</i> WR10 was previously isolated from the root of wheat (<i>Triticum aestivum</i> L.). In this study, the strain WR10 was used for relieving abiotic stresses in wheat under low phosphorus and high s...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/7/11/508 |
| id |
doaj-320fc979907548e683b2069b6a41bc8e |
|---|---|
| record_format |
Article |
| spelling |
doaj-320fc979907548e683b2069b6a41bc8e2020-11-24T21:56:57ZengMDPI AGMicroorganisms2076-26072019-10-0171150810.3390/microorganisms7110508microorganisms7110508Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity StressesZonghao Yue0Yihao Shen1Yanjuan Chen2Anwen Liang3Cuiwei Chu4Can Chen5Zhongke Sun6College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaCollege of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaSchool of Mechanical and Electrical Engineering, Zhoukou Normal University, Zhoukou 466001, ChinaCollege of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaCollege of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaCollege of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaCollege of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou 466001, ChinaAn indole−3−acetic acid producing <i>Bacillus altitudinis</i> WR10 was previously isolated from the root of wheat (<i>Triticum aestivum</i> L.). In this study, the strain WR10 was used for relieving abiotic stresses in wheat under low phosphorus and high saline in hydroponic co-culture models. Significantly, strain WR10 improved wheat seed relative germination rate under salinity stress (200/400 mM NaCl) and the root dry weight in wheat seedlings under phosphorus stress (10 μM KH<sub>2</sub>PO<sub>3</sub>) when insoluble phosphates are available. To provide insights into its abiotic stress-alleviating properties, the strain was characterized further. WR10 grows well under different culture conditions. Particularly, WR10 resists salt (12% NaCl) and hydrolyzes both inorganic and organic insoluble phosphates. WR10 uses many plant-derived substrates as sole carbon and energy sources. It produces catalase, amylase, phosphatase, phytase, reductase, and 1−aminocyclopropane−1−carboxylate (ACC) deaminase. In addition, WR10 possesses long peritrichous flagella, and its biofilm formation, as well as phytase production, is induced by abiotic stresses. Overall, the salinity-alleviating property of WR10 in wheat can be attributed to its inherent tolerance to NaCl, formation of biofilm, and production of enzymes, like catalase, amylase, and ACC deaminase. Meanwhile, <i>B. altitudinis</i> WR10 reduces low-phosphorus stress in wheat by production of phosphatases and phytases in the presence of insoluble phosphates.https://www.mdpi.com/2076-2607/7/11/508<i>bacillus altitudinis</i>wheatendophyteabiotic stressplant growth-promoting bacteria |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Zonghao Yue Yihao Shen Yanjuan Chen Anwen Liang Cuiwei Chu Can Chen Zhongke Sun |
| spellingShingle |
Zonghao Yue Yihao Shen Yanjuan Chen Anwen Liang Cuiwei Chu Can Chen Zhongke Sun Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses Microorganisms <i>bacillus altitudinis</i> wheat endophyte abiotic stress plant growth-promoting bacteria |
| author_facet |
Zonghao Yue Yihao Shen Yanjuan Chen Anwen Liang Cuiwei Chu Can Chen Zhongke Sun |
| author_sort |
Zonghao Yue |
| title |
Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses |
| title_short |
Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses |
| title_full |
Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses |
| title_fullStr |
Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses |
| title_full_unstemmed |
Microbiological Insights into the Stress-Alleviating Property of an Endophytic <i>Bacillus altitudinis</i> WR10 in Wheat under Low-Phosphorus and High-Salinity Stresses |
| title_sort |
microbiological insights into the stress-alleviating property of an endophytic <i>bacillus altitudinis</i> wr10 in wheat under low-phosphorus and high-salinity stresses |
| publisher |
MDPI AG |
| series |
Microorganisms |
| issn |
2076-2607 |
| publishDate |
2019-10-01 |
| description |
An indole−3−acetic acid producing <i>Bacillus altitudinis</i> WR10 was previously isolated from the root of wheat (<i>Triticum aestivum</i> L.). In this study, the strain WR10 was used for relieving abiotic stresses in wheat under low phosphorus and high saline in hydroponic co-culture models. Significantly, strain WR10 improved wheat seed relative germination rate under salinity stress (200/400 mM NaCl) and the root dry weight in wheat seedlings under phosphorus stress (10 μM KH<sub>2</sub>PO<sub>3</sub>) when insoluble phosphates are available. To provide insights into its abiotic stress-alleviating properties, the strain was characterized further. WR10 grows well under different culture conditions. Particularly, WR10 resists salt (12% NaCl) and hydrolyzes both inorganic and organic insoluble phosphates. WR10 uses many plant-derived substrates as sole carbon and energy sources. It produces catalase, amylase, phosphatase, phytase, reductase, and 1−aminocyclopropane−1−carboxylate (ACC) deaminase. In addition, WR10 possesses long peritrichous flagella, and its biofilm formation, as well as phytase production, is induced by abiotic stresses. Overall, the salinity-alleviating property of WR10 in wheat can be attributed to its inherent tolerance to NaCl, formation of biofilm, and production of enzymes, like catalase, amylase, and ACC deaminase. Meanwhile, <i>B. altitudinis</i> WR10 reduces low-phosphorus stress in wheat by production of phosphatases and phytases in the presence of insoluble phosphates. |
| topic |
<i>bacillus altitudinis</i> wheat endophyte abiotic stress plant growth-promoting bacteria |
| url |
https://www.mdpi.com/2076-2607/7/11/508 |
| work_keys_str_mv |
AT zonghaoyue microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT yihaoshen microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT yanjuanchen microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT anwenliang microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT cuiweichu microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT canchen microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses AT zhongkesun microbiologicalinsightsintothestressalleviatingpropertyofanendophyticibacillusaltitudinisiwr10inwheatunderlowphosphorusandhighsalinitystresses |
| _version_ |
1725856235968790528 |