First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis

First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis

Background/Purpose: In Egypt, there is a scarcity of data concerning Naegleria (N.) family, with a shortage of phylogenetic studies. This study's aim was molecular detection, sequencing and phylogenetic analysis of morphologically identified Nagleria and to determine natural seasonal distributi...

Full description

Bibliographic Details
Main Authors: Ayman A. El-Badry, Sayeda M. Aufy, Eman S. El-Wakil, Enas M. Rizk, Soheir S. Mahmoud, Nahed Y. Taha
Format: Article
Language:English
Published: Elsevier 2020-04-01
Series:Journal of Microbiology, Immunology and Infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1684118218302081
id doaj-320ee2011e884218a61fbc36802cc1d4
record_format Article
spelling doaj-320ee2011e884218a61fbc36802cc1d42020-11-25T02:05:24ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822020-04-01532259265First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysisAyman A. El-Badry0Sayeda M. Aufy1Eman S. El-Wakil2Enas M. Rizk3Soheir S. Mahmoud4Nahed Y. Taha5Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia; Corresponding author. Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, 31451, B.O.Box: 2114, Saudi Arabia. Fax: +966 13 3330220.Medical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, EgyptParasitology Department, Theodor Bilharz Research Institute, Giza, EgyptMedical Parasitology Department, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, EgyptParasitology Department, Theodor Bilharz Research Institute, Giza, EgyptMicrobiology Department, Theodor Bilharz Research Institute, Giza, EgyptBackground/Purpose: In Egypt, there is a scarcity of data concerning Naegleria (N.) family, with a shortage of phylogenetic studies. This study's aim was molecular detection, sequencing and phylogenetic analysis of morphologically identified Nagleria and to determine natural seasonal distribution of Nagleria species in water sources of Greater Cairo, Egypt. Methods: A total of 120 water samples were collected during each season over a year. Every water sample was filtrated and cultured on non-nutrient agar (NNA). Morphologically positive Nagleria-like isolates were subjected to Nagleria genus and species-specific PCR targeting rDNA gene, PCR products were sequenced and obtained sequences were phylogenetic analyzed. Results: Nile River water was the only source found to contained Naegleria. For the first time in Egypt, Vahlkampfia ciguana and the Naegleria species N.australiensis, N.philippinensis and N.neojejuensis were identified from the Nile water. The pathogenic Naegleria fowleri, previously reported in Egypt, was however not detected in this study. Conclusion: Interestingly, there were no seasonal variations in prevalence of Naegleria spp.; yet, there was seasonal diversity in the water samples of the same site. These newly discovered Vahlkampfiidae in Egyptian aquatic environments indicate the need for further phylogenetic investigations using bigger sample sizes in order to determine their potential risk for human health. Keywords: Naegleria australiensis, Naegleria philippinensis, Naegleria neojejuensis, Vahlkampfia ciguana, Phylogenetic analysis, Egypthttp://www.sciencedirect.com/science/article/pii/S1684118218302081
collection DOAJ
language English
format Article
sources DOAJ
author Ayman A. El-Badry
Sayeda M. Aufy
Eman S. El-Wakil
Enas M. Rizk
Soheir S. Mahmoud
Nahed Y. Taha
spellingShingle Ayman A. El-Badry
Sayeda M. Aufy
Eman S. El-Wakil
Enas M. Rizk
Soheir S. Mahmoud
Nahed Y. Taha
First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
Journal of Microbiology, Immunology and Infection
author_facet Ayman A. El-Badry
Sayeda M. Aufy
Eman S. El-Wakil
Enas M. Rizk
Soheir S. Mahmoud
Nahed Y. Taha
author_sort Ayman A. El-Badry
title First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
title_short First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
title_full First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
title_fullStr First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
title_full_unstemmed First identification of Naegleria species and Vahlkampfia ciguana in Nile water, Cairo, Egypt: Seasonal morphology and phylogenetic analysis
title_sort first identification of naegleria species and vahlkampfia ciguana in nile water, cairo, egypt: seasonal morphology and phylogenetic analysis
publisher Elsevier
series Journal of Microbiology, Immunology and Infection
issn 1684-1182
publishDate 2020-04-01
description Background/Purpose: In Egypt, there is a scarcity of data concerning Naegleria (N.) family, with a shortage of phylogenetic studies. This study's aim was molecular detection, sequencing and phylogenetic analysis of morphologically identified Nagleria and to determine natural seasonal distribution of Nagleria species in water sources of Greater Cairo, Egypt. Methods: A total of 120 water samples were collected during each season over a year. Every water sample was filtrated and cultured on non-nutrient agar (NNA). Morphologically positive Nagleria-like isolates were subjected to Nagleria genus and species-specific PCR targeting rDNA gene, PCR products were sequenced and obtained sequences were phylogenetic analyzed. Results: Nile River water was the only source found to contained Naegleria. For the first time in Egypt, Vahlkampfia ciguana and the Naegleria species N.australiensis, N.philippinensis and N.neojejuensis were identified from the Nile water. The pathogenic Naegleria fowleri, previously reported in Egypt, was however not detected in this study. Conclusion: Interestingly, there were no seasonal variations in prevalence of Naegleria spp.; yet, there was seasonal diversity in the water samples of the same site. These newly discovered Vahlkampfiidae in Egyptian aquatic environments indicate the need for further phylogenetic investigations using bigger sample sizes in order to determine their potential risk for human health. Keywords: Naegleria australiensis, Naegleria philippinensis, Naegleria neojejuensis, Vahlkampfia ciguana, Phylogenetic analysis, Egypt
url http://www.sciencedirect.com/science/article/pii/S1684118218302081
work_keys_str_mv AT aymanaelbadry firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
AT sayedamaufy firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
AT emanselwakil firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
AT enasmrizk firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
AT soheirsmahmoud firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
AT nahedytaha firstidentificationofnaegleriaspeciesandvahlkampfiaciguanainnilewatercairoegyptseasonalmorphologyandphylogeneticanalysis
_version_ 1724938186817273856

Similar Items