Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing

A novel cross-linked honey hydrogel dressing was developed by incorporating Malaysian honey into hydrogel dressing formulation, cross-linked and sterilized using electron beam irradiation (25 kGy). In this study, the physical properties of the prepared honey hydrogel and its wound healing efficacy o...

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Main Authors: Rozaini Mohd Zohdi, Zuki Abu Bakar Zakaria, Norimah Yusof, Noordin Mohamed Mustapha, Muhammad Nazrul Hakim Abdullah
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2012/843025
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spelling doaj-31d8aa6eb9da43e198cf49887b06aae02020-11-24T23:28:36ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/843025843025Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound DressingRozaini Mohd Zohdi0Zuki Abu Bakar Zakaria1Norimah Yusof2Noordin Mohamed Mustapha3Muhammad Nazrul Hakim Abdullah4Department of Life Sciences, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, MalaysiaDepartment of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDivision of Agrotechnology and Biosciences, Malaysian Nuclear Agency, Bangi, 43000 Kajang, Selangor, MalaysiaDepartment of Veterinary Microbiology and Pathology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaA novel cross-linked honey hydrogel dressing was developed by incorporating Malaysian honey into hydrogel dressing formulation, cross-linked and sterilized using electron beam irradiation (25 kGy). In this study, the physical properties of the prepared honey hydrogel and its wound healing efficacy on deep partial thickness burn wounds in rats were assessed. Skin samples were taken at 7, 14, 21, and 28 days after burn for histopathological and molecular evaluations. Application of honey hydrogel dressings significantly enhanced (P<0.05) wound closure and accelerated the rate of re-epithelialization as compared to control hydrogel and OpSite film dressing. A significant decrease in inflammatory response was observed in honey hydrogel treated wounds as early as 7 days after burn (P<0.05). Semiquantitative analysis using RT-PCR revealed that treatment with honey hydrogel significantly (P<0.05) suppressed the expression of proinflammatory cytokines (IL-1α, IL-1β, and IL-6). The present study substantiates the potential efficacy of honey hydrogel dressings in accelerating burn wound healing.http://dx.doi.org/10.1155/2012/843025
collection DOAJ
language English
format Article
sources DOAJ
author Rozaini Mohd Zohdi
Zuki Abu Bakar Zakaria
Norimah Yusof
Noordin Mohamed Mustapha
Muhammad Nazrul Hakim Abdullah
spellingShingle Rozaini Mohd Zohdi
Zuki Abu Bakar Zakaria
Norimah Yusof
Noordin Mohamed Mustapha
Muhammad Nazrul Hakim Abdullah
Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
Evidence-Based Complementary and Alternative Medicine
author_facet Rozaini Mohd Zohdi
Zuki Abu Bakar Zakaria
Norimah Yusof
Noordin Mohamed Mustapha
Muhammad Nazrul Hakim Abdullah
author_sort Rozaini Mohd Zohdi
title Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
title_short Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
title_full Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
title_fullStr Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
title_full_unstemmed Gelam (Melaleuca spp.) Honey-Based Hydrogel as Burn Wound Dressing
title_sort gelam (melaleuca spp.) honey-based hydrogel as burn wound dressing
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2012-01-01
description A novel cross-linked honey hydrogel dressing was developed by incorporating Malaysian honey into hydrogel dressing formulation, cross-linked and sterilized using electron beam irradiation (25 kGy). In this study, the physical properties of the prepared honey hydrogel and its wound healing efficacy on deep partial thickness burn wounds in rats were assessed. Skin samples were taken at 7, 14, 21, and 28 days after burn for histopathological and molecular evaluations. Application of honey hydrogel dressings significantly enhanced (P<0.05) wound closure and accelerated the rate of re-epithelialization as compared to control hydrogel and OpSite film dressing. A significant decrease in inflammatory response was observed in honey hydrogel treated wounds as early as 7 days after burn (P<0.05). Semiquantitative analysis using RT-PCR revealed that treatment with honey hydrogel significantly (P<0.05) suppressed the expression of proinflammatory cytokines (IL-1α, IL-1β, and IL-6). The present study substantiates the potential efficacy of honey hydrogel dressings in accelerating burn wound healing.
url http://dx.doi.org/10.1155/2012/843025
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