Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice

Chronic stress exposure in adolescence can lead to a lasting change in stress responsiveness later in life and is associated with increased mental health issues in adulthood. Here we investigate whether the Chronic Social Instability (CSI) paradigm influences the behavioral and molecular responses t...

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Main Authors: Oliver Sturman, Lukas von Ziegler, Mattia Privitera, Rebecca Waag, Sian Duss, Yannick Vermeiren, Letizia Giovagnoli, Peter de Deyn, Johannes Bohacek
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Neurobiology of Stress
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352289521000965
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language English
format Article
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author Oliver Sturman
Lukas von Ziegler
Mattia Privitera
Rebecca Waag
Sian Duss
Yannick Vermeiren
Letizia Giovagnoli
Peter de Deyn
Johannes Bohacek
spellingShingle Oliver Sturman
Lukas von Ziegler
Mattia Privitera
Rebecca Waag
Sian Duss
Yannick Vermeiren
Letizia Giovagnoli
Peter de Deyn
Johannes Bohacek
Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
Neurobiology of Stress
Acute stress
Noradrenaline
Transcriptome
Hippocampus
Social stress
Adolescence
author_facet Oliver Sturman
Lukas von Ziegler
Mattia Privitera
Rebecca Waag
Sian Duss
Yannick Vermeiren
Letizia Giovagnoli
Peter de Deyn
Johannes Bohacek
author_sort Oliver Sturman
title Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
title_short Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
title_full Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
title_fullStr Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
title_full_unstemmed Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 mice
title_sort chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male c57bl/6 mice
publisher Elsevier
series Neurobiology of Stress
issn 2352-2895
publishDate 2021-11-01
description Chronic stress exposure in adolescence can lead to a lasting change in stress responsiveness later in life and is associated with increased mental health issues in adulthood. Here we investigate whether the Chronic Social Instability (CSI) paradigm influences the behavioral and molecular responses to novel acute stressors in mice, and whether it alters physiological responses influenced by the noradrenergic system. Using large cohorts of mice, we show that CSI mice display a persistent increase in exploratory behaviors in the open field test alongside small but widespread transcriptional changes in the ventral hippocampus. However, both the transcriptomic and behavioral responses to novel acute stressors are indistinguishable between groups. In addition, the pupillometric response to a tail shock, known to be mediated by the noradrenergic system, remains unaltered in CSI mice. Ultra-high performance liquid chromatography analysis of monoaminergic neurotransmitter levels in the ventral hippocampus also shows no differences between control or CSI mice at baseline or in response to acute stress. We conclude that CSI exposure during adolescence leads to persistent changes in exploratory behavior and gene expression in the hippocampus, but it does not alter the response to acute stress in adulthood and is unlikely to alter the function of the noradrenergic system.
topic Acute stress
Noradrenaline
Transcriptome
Hippocampus
Social stress
Adolescence
url http://www.sciencedirect.com/science/article/pii/S2352289521000965
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spelling doaj-31893aaaaf1e4714a01718762a2a5bc42021-09-09T04:28:28ZengElsevierNeurobiology of Stress2352-28952021-11-0115100388Chronic adolescent stress increases exploratory behavior but does not appear to change the acute stress response in adult male C57BL/6 miceOliver Sturman0Lukas von Ziegler1Mattia Privitera2Rebecca Waag3Sian Duss4Yannick Vermeiren5Letizia Giovagnoli6Peter de Deyn7Johannes Bohacek8Laboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandDepartment of Biomedical Sciences, Laboratory of Neurochemistry and Behavior, Institute Born-Bunge, University of Antwerp, Wilrijk, Antwerp, Belgium; Division of Human Nutrition and Health, Chair Group of Nutritional Biology, Wageningen University & Research, Wageningen, Netherlands; Faculty of Medicine & Health Sciences, Translational Neurosciences, University of Antwerp, Antwerp, BelgiumLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, SwitzerlandDepartment of Biomedical Sciences, Laboratory of Neurochemistry and Behavior, Institute Born-Bunge, University of Antwerp, Wilrijk, Antwerp, Belgium; Department of Neurology and Alzheimer Center, University of Groningen and University Medical Center Groningen (UMCG), Groningen, Netherlands; Department of Neurology, Memory Clinic of Hospital Network Antwerp (ZNA) Middelheim and Hoge Beuken, Antwerp, BelgiumLaboratory of Molecular and Behavioral Neuroscience, Institute for Neuroscience, Department of Health Sciences and Technology, ETH, Zurich, Switzerland; Neuroscience Center Zurich, ETH Zurich and University of Zurich, Switzerland; Corresponding author. ETH Zurich, Insitute for Neuroscience, Winterthurerstrasse 190, 8057 Zurich, Switzerland.Chronic stress exposure in adolescence can lead to a lasting change in stress responsiveness later in life and is associated with increased mental health issues in adulthood. Here we investigate whether the Chronic Social Instability (CSI) paradigm influences the behavioral and molecular responses to novel acute stressors in mice, and whether it alters physiological responses influenced by the noradrenergic system. Using large cohorts of mice, we show that CSI mice display a persistent increase in exploratory behaviors in the open field test alongside small but widespread transcriptional changes in the ventral hippocampus. However, both the transcriptomic and behavioral responses to novel acute stressors are indistinguishable between groups. In addition, the pupillometric response to a tail shock, known to be mediated by the noradrenergic system, remains unaltered in CSI mice. Ultra-high performance liquid chromatography analysis of monoaminergic neurotransmitter levels in the ventral hippocampus also shows no differences between control or CSI mice at baseline or in response to acute stress. We conclude that CSI exposure during adolescence leads to persistent changes in exploratory behavior and gene expression in the hippocampus, but it does not alter the response to acute stress in adulthood and is unlikely to alter the function of the noradrenergic system.http://www.sciencedirect.com/science/article/pii/S2352289521000965Acute stressNoradrenalineTranscriptomeHippocampusSocial stressAdolescence