| Summary: | This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (R<sub>ct</sub>) value for the different bacteria concentrations under investigation. As a proof-of-concept, <i>Streptococcus mutans</i> (<i>S. mutans</i>) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 10<sup>3</sup> CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.
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