Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease

Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our resul...

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Main Authors: Lingyan Shi, Luyao Lu, George Harvey, Thomas Harvey, Adrián Rodríguez-Contreras, Robert R. Alfano
Format: Article
Language:English
Published: Nature Publishing Group 2017-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-02673-5
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spelling doaj-3147d78c140b4a9dbb2b094ac76b91a22020-12-08T00:53:02ZengNature Publishing GroupScientific Reports2045-23222017-06-01711710.1038/s41598-017-02673-5Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s DiseaseLingyan Shi0Luyao Lu1George Harvey2Thomas Harvey3Adrián Rodríguez-Contreras4Robert R. Alfano5Institute for Ultrafast Spectroscopy and Lasers, the City College of the City University of New YorkDepartment of Biomedical Engineering, Wenzhou Medical UniversityRiverdale Country SchoolRiverdale Country SchoolInstitute for Ultrafast Spectroscopy and Lasers, the City College of the City University of New YorkInstitute for Ultrafast Spectroscopy and Lasers, the City College of the City University of New YorkAbstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.https://doi.org/10.1038/s41598-017-02673-5
collection DOAJ
language English
format Article
sources DOAJ
author Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
spellingShingle Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
Scientific Reports
author_facet Lingyan Shi
Luyao Lu
George Harvey
Thomas Harvey
Adrián Rodríguez-Contreras
Robert R. Alfano
author_sort Lingyan Shi
title Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_short Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_fullStr Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_full_unstemmed Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer’s Disease
title_sort label-free fluorescence spectroscopy for detecting key biomolecules in brain tissue from a mouse model of alzheimer’s disease
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2017-06-01
description Abstract In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer’s disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
url https://doi.org/10.1038/s41598-017-02673-5
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