Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake

Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake

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Urmia Lake is one of the largest hypersaline lakes in the world. Water evaporation and saturation cause a lot of salt crystals formation on the lake beach. In this study, extremely halophilic strains were isolated from salt crystals that formed in distinct regions of lake. The isolation was performe...

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Main Authors: Gholamreza Zarrini, Rana Rahmani, Manica Negahdaripour, Miald Mohkam, Younes Ghasemi
Format: Article
Language:English
Published: Shiraz University of Medical Sciences 2018-06-01
Series:Trends in Pharmaceutical Sciences
Online Access:http://tips.sums.ac.ir/article_42257_a926b046e86da7066b19f765aec14bd0.pdf
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spelling doaj-3142f42023c1497e96648f2f0cfdaa112020-11-25T01:35:55ZengShiraz University of Medical SciencesTrends in Pharmaceutical Sciences2423-56522018-06-014242257Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia LakeGholamreza Zarrini0Rana Rahmani1Manica NegahdaripourMiald MohkamYounes Ghasemi2Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, IranDepartment of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, IranDepartment of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.Urmia Lake is one of the largest hypersaline lakes in the world. Water evaporation and saturation cause a lot of salt crystals formation on the lake beach. In this study, extremely halophilic strains were isolated from salt crystals that formed in distinct regions of lake. The isolation was performed by means of modified Marine agar medium and their DNA were extracted and amplified by PCR using universal primers that amplify archaeal 16S rDNA. The amplified archeal DNA fragments were purified, and were subjected to 16S rRNA gene sequencing analysis which was compared to known sequences by a Blast search at NCBI (National Center for Biological Information). Similarity analysis based on 16S rRNA gene sequences of all isolates indicated that the archaeal isolates belong to three different halophilic genera of euryarcheota: Halorubrum, Haloarcula and Halobacterium. These extreme halophilc archaea can be used as a potential source of new therapeutic metabolites and enzymes as well as antibiotic compounds along with novel biotechnological applications.http://tips.sums.ac.ir/article_42257_a926b046e86da7066b19f765aec14bd0.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Gholamreza Zarrini
Rana Rahmani
Manica Negahdaripour
Miald Mohkam
Younes Ghasemi
spellingShingle Gholamreza Zarrini
Rana Rahmani
Manica Negahdaripour
Miald Mohkam
Younes Ghasemi
Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
Trends in Pharmaceutical Sciences
author_facet Gholamreza Zarrini
Rana Rahmani
Manica Negahdaripour
Miald Mohkam
Younes Ghasemi
author_sort Gholamreza Zarrini
title Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
title_short Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
title_full Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
title_fullStr Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
title_full_unstemmed Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake
title_sort isolation and identification of culturable aerobic halophilic archaea associated with salt crystals from urmia lake
publisher Shiraz University of Medical Sciences
series Trends in Pharmaceutical Sciences
issn 2423-5652
publishDate 2018-06-01
description Urmia Lake is one of the largest hypersaline lakes in the world. Water evaporation and saturation cause a lot of salt crystals formation on the lake beach. In this study, extremely halophilic strains were isolated from salt crystals that formed in distinct regions of lake. The isolation was performed by means of modified Marine agar medium and their DNA were extracted and amplified by PCR using universal primers that amplify archaeal 16S rDNA. The amplified archeal DNA fragments were purified, and were subjected to 16S rRNA gene sequencing analysis which was compared to known sequences by a Blast search at NCBI (National Center for Biological Information). Similarity analysis based on 16S rRNA gene sequences of all isolates indicated that the archaeal isolates belong to three different halophilic genera of euryarcheota: Halorubrum, Haloarcula and Halobacterium. These extreme halophilc archaea can be used as a potential source of new therapeutic metabolites and enzymes as well as antibiotic compounds along with novel biotechnological applications.
url http://tips.sums.ac.ir/article_42257_a926b046e86da7066b19f765aec14bd0.pdf
work_keys_str_mv AT gholamrezazarrini isolationandidentificationofculturableaerobichalophilicarchaeaassociatedwithsaltcrystalsfromurmialake
AT ranarahmani isolationandidentificationofculturableaerobichalophilicarchaeaassociatedwithsaltcrystalsfromurmialake
AT manicanegahdaripour isolationandidentificationofculturableaerobichalophilicarchaeaassociatedwithsaltcrystalsfromurmialake
AT mialdmohkam isolationandidentificationofculturableaerobichalophilicarchaeaassociatedwithsaltcrystalsfromurmialake
AT younesghasemi isolationandidentificationofculturableaerobichalophilicarchaeaassociatedwithsaltcrystalsfromurmialake
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