Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection

• Main Authors: Taketo Inoue, Sayumi Taguchi, Kayoko Hirao, Yoshiko Tsujimoto, Shuji Yamamoto, Mikiko Uemura, Kazunori Miyazaki, Yoshiki Yamashita
• Format: Article
• Language: English
• Published: Wiley 2018-07-01
• Series: Reproductive Medicine and Biology
• Subjects: gonadotropin‐releasing hormone; infertility; intracytoplasmic sperm injection; ovarian follicle; ovulation induction
• Online Access: https://doi.org/10.1002/rmb2.12102
• ISSN: 1445-5781; 1447-0578

Abstract Purpose The authors previously revealed the association of the follicular fluid (FF) volume with oolemma stretchability following the gonadotropin‐releasing hormone (GnRH) antagonist protocol during intracytoplasmic sperm injection (ICSI). However, the impact of the GnRH agonist protocol on oolemma stretchability remains unclear. Methods Data that were obtained from 74 ICSI cycles were reviewed retrospectively. Controlled ovarian stimulation was performed in accordance with the short GnRH agonist protocol. Each follicle was individually aspirated and assigned to one of six groups, according to the FF volume. The oolemma stretchability during ICSI was evaluated by using a mechanical stimulus for oolemma penetration; that is, oolemma penetration with or without aspiration (high vs low stretchability, respectively). Results The incidence of low oolemma stretchability was significantly higher in the <1.0 mL group than that in the ≥1.0 mL group. The normal fertilization rate was significantly lower in the <1.0 mL group than that in the 2.0‐<3.0 mL group. The rate of blastocyst development was lower in the <1.0 mL group than that in the 3.0‐<4.0 mL group. Conclusion The FF volume potentially was associated with metaphase II oolemma stretchability, fertilization, and blastocyst development.