Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway
Korean plum (<i>Prunus mume</i> (Siebold) Siebold & Zucc.) has long been used as a health food or herbal medicine in Asia. Previous studies have shown that several plants of the genus <i>Prunus</i> have vasodilatory and antihypertensive effects; we hypothesized that &...
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doaj-3108a18c45974162aa157e1f7a0552bd2020-11-25T02:24:22ZengMDPI AGMolecules1420-30492019-09-012418334010.3390/molecules24183340molecules24183340Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent PathwayCheolmin Jo0Bumjung Kim1Somin Lee2Inhye Ham3Kyungjin Lee4Ho-Young Choi5Department of Herbal Pharmacology, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, KoreaKorean plum (<i>Prunus mume</i> (Siebold) Siebold & Zucc.) has long been used as a health food or herbal medicine in Asia. Previous studies have shown that several plants of the genus <i>Prunus</i> have vasodilatory and antihypertensive effects; we hypothesized that <i>P. mume</i> branches may have a vasorelaxant effect. In this study, we evaluated the effects and action mechanism of 70% ethanol extract of <i>P. mume</i> branch (PMB) on isolated rat aortic rings. Inhibitors such as NG-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester, 1<i>H</i>-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, methylene blue, indomethacin, atropine, tetraethylammonium chloride, glibenclamide, 4-aminopyridine and BaCl<sub>2</sub> were used to investigate the mechanism of vasodilation responsible for the vascular relaxation. PMB (2−30 μg/mL) induced vasorelaxation in the presence of vascular endothelium, and all inhibitors used in this study affected the degree of relaxation. These results suggest that the vasorelaxant effect of PMB is endothelium-dependent and affects the nitric oxide-cyclic guanosine monophosphate pathway, prostacyclin pathway, muscarinic receptor pathway, and potassium channels. Our study explains that PMB may be another approach to hypertension treatment to reduce the burden of cardiovascular disease.https://www.mdpi.com/1420-3049/24/18/3340<i>Prunus mume</i> (Siebold) Siebold &Zucc.vasorelaxationendothelium-dependenthypertensioncardiovascular diseaseKorean plum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheolmin Jo Bumjung Kim Somin Lee Inhye Ham Kyungjin Lee Ho-Young Choi |
spellingShingle |
Cheolmin Jo Bumjung Kim Somin Lee Inhye Ham Kyungjin Lee Ho-Young Choi Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway Molecules <i>Prunus mume</i> (Siebold) Siebold & Zucc. vasorelaxation endothelium-dependent hypertension cardiovascular disease Korean plum |
author_facet |
Cheolmin Jo Bumjung Kim Somin Lee Inhye Ham Kyungjin Lee Ho-Young Choi |
author_sort |
Cheolmin Jo |
title |
Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway |
title_short |
Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway |
title_full |
Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway |
title_fullStr |
Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway |
title_full_unstemmed |
Vasorelaxant Effect of <i>Prunus mume</i> (Siebold) Siebold & Zucc. Branch through the Endothelium-Dependent Pathway |
title_sort |
vasorelaxant effect of <i>prunus mume</i> (siebold) siebold & zucc. branch through the endothelium-dependent pathway |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-09-01 |
description |
Korean plum (<i>Prunus mume</i> (Siebold) Siebold & Zucc.) has long been used as a health food or herbal medicine in Asia. Previous studies have shown that several plants of the genus <i>Prunus</i> have vasodilatory and antihypertensive effects; we hypothesized that <i>P. mume</i> branches may have a vasorelaxant effect. In this study, we evaluated the effects and action mechanism of 70% ethanol extract of <i>P. mume</i> branch (PMB) on isolated rat aortic rings. Inhibitors such as NG-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester, 1<i>H</i>-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, methylene blue, indomethacin, atropine, tetraethylammonium chloride, glibenclamide, 4-aminopyridine and BaCl<sub>2</sub> were used to investigate the mechanism of vasodilation responsible for the vascular relaxation. PMB (2−30 μg/mL) induced vasorelaxation in the presence of vascular endothelium, and all inhibitors used in this study affected the degree of relaxation. These results suggest that the vasorelaxant effect of PMB is endothelium-dependent and affects the nitric oxide-cyclic guanosine monophosphate pathway, prostacyclin pathway, muscarinic receptor pathway, and potassium channels. Our study explains that PMB may be another approach to hypertension treatment to reduce the burden of cardiovascular disease. |
topic |
<i>Prunus mume</i> (Siebold) Siebold & Zucc. vasorelaxation endothelium-dependent hypertension cardiovascular disease Korean plum |
url |
https://www.mdpi.com/1420-3049/24/18/3340 |
work_keys_str_mv |
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