| Summary: | This experiment was conducted to evaluate the chemical composition and standardized ileal amino acid digestibility of corn grain and to use these data to develop prediction equations for estimating total amino acids (TAAs) and standardized ileal digestible amino acids (SIDAAs) for broiler chickens. Four types of corn grains were obtained from different origins (Brazil, Ukraine, Russia, and Iran). Eighty-day-old Ross 308 male broiler chicks were fed a standard diet until day 18, and experimental diets were fed from 19 to 24 D of age. Five dietary treatments consisted of 4 semi-purified diets containing corn from each origin as the only source of amino acid (AA) and a N-free diet for determination of basal endogenous AA losses. Assay diets contained 939 g of test corn/kg. The concentration of crude protein and gross energy ranged from 7.58 to 8.39% (coefficient of variation [CV] = 4.72%) and 4,121 to 4,621 kcal/kg (CV = 5.09%), respectively. There was significant variation among the 4 corn grains in standardized ileal digestibility (SID) for CP, Phe, Leu, Asp, Glu, Ser, Gly, Ala, and Tyr (P ≤ 0.05). The results of linear regression showed that linear prediction equations based on protein content can be used to predict the TAA and SIDAA contents (e.g., TLys = 0.041 × CP, adj R2 = 95.9, standard error of prediction [SEP] = 0.05; SIDLys = 0.0356 × CP, adj R2 = 96, SEP = 0.051). Inclusion of other proximate components of test samples into the regression equation increased the R2 value and decreased the SEP value (e.g., TLys = 0.329 × crude fiber [CF] ‒ 0.209 × Ash, adj R2 = 99.9, SEP = 0.005; SIDLys = ‒1.1591 + 0.836 × CF ‒ 0.055 × Ash, adj R2 = 99.9, SEP = 0.001). The concentration of TAA and SIDAA was highly correlated (adj R2 > 89%) for most AA and showed that the amount of SIDAA could be predicted from its total concentration with a high degree of accuracy (e.g., SIDLys = 0.0023 + 0.861 × TLys, adj R2 = 99.9, SEP = 0.0001). In conclusion, this in vitro assays and equations accurately predicted TAA and SIDAA corn grain samples for broiler chickens and can serve as a reference analysis to develop calibration equations for rapid feed quality evaluation methods such as near-infrared reflectance spectroscopy.
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