Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut

• Main Authors: ENDANG PUDJIHARTATI, SISWANTO, SATRIYAS ILYAS, SUDARSONO
• Format: Article
• Language: English
• Published: Bogor Agricultural University 2006-06-01
• Series: Hayati Journal of Biosciences
• Subjects: Disease response; stem rot disease; endo- and exo-chitinase
• Online Access: http://journal.ipb.ac.id/index.php/hayati/article/viewFile/210/76

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45).