Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus

The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess...

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Main Authors: JD Biller-Takahashi, LS Takahashi, MV Saita, RY Gimbo, EC Urbinati
Format: Article
Language:English
Published: Instituto Internacional de Ecologia
Series:Brazilian Journal of Biology
Subjects:
immune methodology
fish immunology
cellular immunity
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842013000200425&lng=en&tlng=en
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spelling doaj-2fe1f0fe4db74ee0acaac84127e987032020-11-24T22:39:27ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-437573242542910.1590/S1519-69842013000200026S1519-69842013000200425Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicusJD Biller-TakahashiLS TakahashiMV SaitaRY GimboEC UrbinatiThe present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842013000200425&lng=en&tlng=enimmune methodologyfish immunologycellular immunity
collection DOAJ
language English
format Article
sources DOAJ
author JD Biller-Takahashi
LS Takahashi
MV Saita
RY Gimbo
EC Urbinati
spellingShingle JD Biller-Takahashi
LS Takahashi
MV Saita
RY Gimbo
EC Urbinati
Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
Brazilian Journal of Biology
immune methodology
fish immunology
cellular immunity
author_facet JD Biller-Takahashi
LS Takahashi
MV Saita
RY Gimbo
EC Urbinati
author_sort JD Biller-Takahashi
title Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
title_short Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
title_full Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
title_fullStr Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
title_full_unstemmed Leukocytes respiratory burst activity as indicator of innate immunity of pacu Piaractus mesopotamicus
title_sort leukocytes respiratory burst activity as indicator of innate immunity of pacu piaractus mesopotamicus
publisher Instituto Internacional de Ecologia
series Brazilian Journal of Biology
issn 1678-4375
description The present study evaluated the assay to quantify the respiratory burst activity of blood leukocytes of pacu as an indicator of the innate immune system, using the reduction of nitroblue tetrazolium (NBT) to formazan as a measure of the production of reactive oxygen species (ROS). In order to assess the accuracy of the assay, fish were challenged by Aeromonas hydrophila and sampled one week after challenge. The A. hydrophila infection increased the leukocyte respiratory burst activity. The protocol showed a reliable and easy assay, appropriate to determine the respiratory burst activity of blood leukocytes of pacu, a neotropical fish, in the present experimental conditions.
topic immune methodology
fish immunology
cellular immunity
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842013000200425&lng=en&tlng=en
work_keys_str_mv AT jdbillertakahashi leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus
AT lstakahashi leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus
AT mvsaita leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus
AT rygimbo leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus
AT ecurbinati leukocytesrespiratoryburstactivityasindicatorofinnateimmunityofpacupiaractusmesopotamicus
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