Exposure of rainbow trout (Oncorhynchus mykiss) to magnetite (Fe3O4) nanoparticles in simplified food chain: Study on ultrastructural characterization

• Main Authors: Nargiz J. Agayeva, Fuad H. Rzayev, Eldar K. Gasimov, Chingiz A. Mamedov, Ismat S. Ahmadov, Narmina A. Sadigova, Ameer Khusro, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu
• Format: Article
• Language: English
• Published: Elsevier 2020-12-01
• Series: Saudi Journal of Biological Sciences
• Subjects: Aquatic ecosystem; Bioaccumulation; Fe3O4 nanoparticles; Microscopic analyses; Oncorhynchus mykiss
• Online Access: http://www.sciencedirect.com/science/article/pii/S1319562X20304393

The widespread exposure of metallic nanoparticles to the aquatic ecosystem and its adverse impact on human life is the colossal concern worldwide. In view of this, this context was investigated to analyze microscopically the bioaccumulation and localization of magnetite (Fe3O4) nanoparticles in the cellular organelles of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in aquatic conditions. Initially, Fe3O4 nanoparticles were absorbed on to Elodea (Elodea canadensis) and fed to molluscs (Melanopsis praemorsa). Fish were fed with the same molluscs, and then the intestines and liver were examined using light and transmission electron microscopy. Results showed that nanoparticles were present in the cytoplasm and other organelles of cells (mitochondrion and lysosome) by absorbing through microvilli of the epithelial cells of the tunica mucosa in the intestine. Further, nanoparticles passed through the vessels of the lamina propria of the tunica mucosa and reached to the sinusoids of the liver via blood circulation. It was then accumulated from the endothelium of the sinusoid to the cytoplasm of liver hepatocytes and to mitochondria and lysosome. The accumulation of nanoparticles in the epithelial cells, cytoplasm, mitochondria, and lysosome revealed the degree of transparency of the pattern with slight hesitation. In summary, this investigation contributed towards the understanding of the physiological effects of Fe3O4 nanoparticles on O. mykiss, which ascertains essentiality for sustainable development of nanobiotechnology in the aquatic ecosystem.