EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING E. COLI CONTAMINATION OF CHICKEN MEAT IN THE IRISH RETAIL MARKET

• Main Authors: Dearbháile Morris, Catherine Ludden, Enda Burke, Martin Cormican
• Format: Article
• Language: English
• Published: Slovak University of Agriculture 2014-04-01
• Series: Journal of Microbiology, Biotechnology and Food Sciences
• Subjects: Extended spectrum beta-lactamase; E.coli; food safety; molecular epidemiology
• Online Access: http://www.jmbfs.org/wp-content/uploads/2014/03/jmbfs_0638_morris.pdf
• ISSN: 1338-5178

Animals represent potential reservoirs for the dissemination of antimicrobial resistance. Twenty domestically produced chicken meat samples were collected from 19 retail outlets in Ireland, inoculated into Bolton broth and cultured on modified charcoal cefoperazone deoxycholate (mCCDA) and Preston agars. Selected representative coliforms included 16 E.coli and 4 Pseudomonas aeruginosa. All E.coli isolates were confirmed as ESBL producers, 15 isolates harbored a blaCTX-M group-1 gene, and none belonged to the E.coli 025b:H4-ST131 clonal group. Pulsed field gel electrophoresis (PFGE) analysis identified 13 distinct pulsed field profiles and comparison with more than 300 human clinical isolates of ESBL producing E. coli did not reveal any similarities. ESBL producing E. coli were detected on retail meats in the Irish market place. Although no similarity was apparent between poultry and human isolates this does not preclude a role for ESBL-producing E.coli in meat in dissemination of antimicrobial resistance.