Detection and Molecular Characterization of Grapevine Virus A in Jordan
In a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995),...
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Firenze University Press
2004-12-01
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| Series: | Phytopathologia Mediterranea |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5091 |
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doaj-2fac7e6a930f40598d36328b0aa55abe2020-11-25T03:50:03ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952004-12-0143310.14601/Phytopathol_Mediterr-17641760Detection and Molecular Characterization of Grapevine Virus A in JordanG. AnfokaW. ShahrourM.K. NakhlaIn a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995), a portion of the coat protein gene of the virus was amplified by IC-RT-PCR and RT-PCR, using leaf extracts and RNA extracted from infected grapevines respectively. After cloning and sequencing the coat protein gene of the Jordanian isolate of GVA (GVA-Jo), the sequence of the amplified product was compared with sequences of other GVA isolates from different countries.https://oajournals.fupress.net/index.php/pm/article/view/5091 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
G. Anfoka W. Shahrour M.K. Nakhla |
| spellingShingle |
G. Anfoka W. Shahrour M.K. Nakhla Detection and Molecular Characterization of Grapevine Virus A in Jordan Phytopathologia Mediterranea |
| author_facet |
G. Anfoka W. Shahrour M.K. Nakhla |
| author_sort |
G. Anfoka |
| title |
Detection and Molecular Characterization of Grapevine Virus A in Jordan |
| title_short |
Detection and Molecular Characterization of Grapevine Virus A in Jordan |
| title_full |
Detection and Molecular Characterization of Grapevine Virus A in Jordan |
| title_fullStr |
Detection and Molecular Characterization of Grapevine Virus A in Jordan |
| title_full_unstemmed |
Detection and Molecular Characterization of Grapevine Virus A in Jordan |
| title_sort |
detection and molecular characterization of grapevine virus a in jordan |
| publisher |
Firenze University Press |
| series |
Phytopathologia Mediterranea |
| issn |
0031-9465 1593-2095 |
| publishDate |
2004-12-01 |
| description |
In a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was
detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found
that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995), a portion of the coat
protein gene of the virus was amplified by IC-RT-PCR and RT-PCR, using leaf extracts and RNA extracted from
infected grapevines respectively. After cloning and sequencing the coat protein gene of the Jordanian isolate of GVA
(GVA-Jo), the sequence of the amplified product was compared with sequences of other GVA isolates from different
countries. |
| url |
https://oajournals.fupress.net/index.php/pm/article/view/5091 |
| work_keys_str_mv |
AT ganfoka detectionandmolecularcharacterizationofgrapevinevirusainjordan AT wshahrour detectionandmolecularcharacterizationofgrapevinevirusainjordan AT mknakhla detectionandmolecularcharacterizationofgrapevinevirusainjordan |
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