Detection and Molecular Characterization of Grapevine Virus A in Jordan
In a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995),...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2004-12-01
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| Series: | Phytopathologia Mediterranea |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/5091 |
| Summary: | In a study on grapevines in Jordan conducted between 2002 and 2003, grapevine virus A (GVA) was
detected in all areas where grapevines were planted. DAS-ELISA analysis of samples from symptomatic trees found
that 16.1% of samples were infected with GVA. Using a GVA- specific primer pair (H587/C995), a portion of the coat
protein gene of the virus was amplified by IC-RT-PCR and RT-PCR, using leaf extracts and RNA extracted from
infected grapevines respectively. After cloning and sequencing the coat protein gene of the Jordanian isolate of GVA
(GVA-Jo), the sequence of the amplified product was compared with sequences of other GVA isolates from different
countries. |
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| ISSN: | 0031-9465 1593-2095 |