Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
The influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. T...
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doaj-2f92afe362ae48aca6b9fdf117ebbe992020-11-25T02:09:34ZengElsevierHeliyon2405-84402019-05-0155e01657Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelaninAl Shimaa Gamal Shalaby0Tamer I.M. Ragab1Mohamed M.I. Helal2Mona A. Esawy3Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptCorresponding author.; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptThe influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. The tyrosinase enzymes were optimized by studying different environmental and nutritional factors. The maximum monophenolase and diphenolase productivity were obtained at 60 °C, pH9, Cu2+(0.01g), liver extract (1 g/L) and the oxygen level fixed at 20%. Also, the mannose as a carbon source increased the monophenolase production 6.2 times. For the first time, two types of eumelanin were extracted by hydrochloric acid treatment. The black and brown eumelanin weighed (0.1 g/100 mL and 0.7 g/100 mL respectively) and characterized by using FTIR and UV/Vis spectroscopy techniques. Their morphological structure and its elemental composition were characterized by SEM and EDAX respectively. The black melanin showed promising anticancer activity towards HEPG-2 and HCT-116 cell lines with IC50 values (6.15, 5.54 μg) compared to Doxorubicin (4.05, 4.45 μg) respectively.http://www.sciencedirect.com/science/article/pii/S2405844018366908Cancer researchMicrobiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Al Shimaa Gamal Shalaby Tamer I.M. Ragab Mohamed M.I. Helal Mona A. Esawy |
spellingShingle |
Al Shimaa Gamal Shalaby Tamer I.M. Ragab Mohamed M.I. Helal Mona A. Esawy Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin Heliyon Cancer research Microbiology |
author_facet |
Al Shimaa Gamal Shalaby Tamer I.M. Ragab Mohamed M.I. Helal Mona A. Esawy |
author_sort |
Al Shimaa Gamal Shalaby |
title |
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin |
title_short |
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin |
title_full |
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin |
title_fullStr |
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin |
title_full_unstemmed |
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin |
title_sort |
optimization of bacillus licheniformis mal tyrosinase: in vitro anticancer activity for brown and black eumelanin |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-05-01 |
description |
The influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. The tyrosinase enzymes were optimized by studying different environmental and nutritional factors. The maximum monophenolase and diphenolase productivity were obtained at 60 °C, pH9, Cu2+(0.01g), liver extract (1 g/L) and the oxygen level fixed at 20%. Also, the mannose as a carbon source increased the monophenolase production 6.2 times. For the first time, two types of eumelanin were extracted by hydrochloric acid treatment. The black and brown eumelanin weighed (0.1 g/100 mL and 0.7 g/100 mL respectively) and characterized by using FTIR and UV/Vis spectroscopy techniques. Their morphological structure and its elemental composition were characterized by SEM and EDAX respectively. The black melanin showed promising anticancer activity towards HEPG-2 and HCT-116 cell lines with IC50 values (6.15, 5.54 μg) compared to Doxorubicin (4.05, 4.45 μg) respectively. |
topic |
Cancer research Microbiology |
url |
http://www.sciencedirect.com/science/article/pii/S2405844018366908 |
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