Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin

The influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. T...

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Main Authors: Al Shimaa Gamal Shalaby, Tamer I.M. Ragab, Mohamed M.I. Helal, Mona A. Esawy
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Heliyon
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844018366908
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spelling doaj-2f92afe362ae48aca6b9fdf117ebbe992020-11-25T02:09:34ZengElsevierHeliyon2405-84402019-05-0155e01657Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelaninAl Shimaa Gamal Shalaby0Tamer I.M. Ragab1Mohamed M.I. Helal2Mona A. Esawy3Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptChemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptCorresponding author.; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, EgyptThe influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. The tyrosinase enzymes were optimized by studying different environmental and nutritional factors. The maximum monophenolase and diphenolase productivity were obtained at 60 °C, pH9, Cu2+(0.01g), liver extract (1 g/L) and the oxygen level fixed at 20%. Also, the mannose as a carbon source increased the monophenolase production 6.2 times. For the first time, two types of eumelanin were extracted by hydrochloric acid treatment. The black and brown eumelanin weighed (0.1 g/100 mL and 0.7 g/100 mL respectively) and characterized by using FTIR and UV/Vis spectroscopy techniques. Their morphological structure and its elemental composition were characterized by SEM and EDAX respectively. The black melanin showed promising anticancer activity towards HEPG-2 and HCT-116 cell lines with IC50 values (6.15, 5.54 μg) compared to Doxorubicin (4.05, 4.45 μg) respectively.http://www.sciencedirect.com/science/article/pii/S2405844018366908Cancer researchMicrobiology
collection DOAJ
language English
format Article
sources DOAJ
author Al Shimaa Gamal Shalaby
Tamer I.M. Ragab
Mohamed M.I. Helal
Mona A. Esawy
spellingShingle Al Shimaa Gamal Shalaby
Tamer I.M. Ragab
Mohamed M.I. Helal
Mona A. Esawy
Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
Heliyon
Cancer research
Microbiology
author_facet Al Shimaa Gamal Shalaby
Tamer I.M. Ragab
Mohamed M.I. Helal
Mona A. Esawy
author_sort Al Shimaa Gamal Shalaby
title Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
title_short Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
title_full Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
title_fullStr Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
title_full_unstemmed Optimization of Bacillus licheniformis MAL tyrosinase: in vitro anticancer activity for brown and black eumelanin
title_sort optimization of bacillus licheniformis mal tyrosinase: in vitro anticancer activity for brown and black eumelanin
publisher Elsevier
series Heliyon
issn 2405-8440
publishDate 2019-05-01
description The influence of tyrosinase in catalyzes/stimulates the eumelanin production was studied. Accordingly, bacterial sp. was isolated and identified as Bacillus licheniformis based on 16S rRNA. It could grow and gave monophenolase and diphenolase productivity in medium contained tyrosin and Cu2+ only. The tyrosinase enzymes were optimized by studying different environmental and nutritional factors. The maximum monophenolase and diphenolase productivity were obtained at 60 °C, pH9, Cu2+(0.01g), liver extract (1 g/L) and the oxygen level fixed at 20%. Also, the mannose as a carbon source increased the monophenolase production 6.2 times. For the first time, two types of eumelanin were extracted by hydrochloric acid treatment. The black and brown eumelanin weighed (0.1 g/100 mL and 0.7 g/100 mL respectively) and characterized by using FTIR and UV/Vis spectroscopy techniques. Their morphological structure and its elemental composition were characterized by SEM and EDAX respectively. The black melanin showed promising anticancer activity towards HEPG-2 and HCT-116 cell lines with IC50 values (6.15, 5.54 μg) compared to Doxorubicin (4.05, 4.45 μg) respectively.
topic Cancer research
Microbiology
url http://www.sciencedirect.com/science/article/pii/S2405844018366908
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