Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers

We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphis...

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Main Authors: Sysoliatin Eugeny Nikolaevich, Anisimova Natalia Vladimirovna, Babak Olga Gennadievna
Format: Article
Language:English
Published: Sciendo 2017-05-01
Series:The EuroBiotech Journal
Online Access:https://doi.org/10.24190/ISSN2564-615X/2017/02.18
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spelling doaj-2f7f2fae56eb44649f17c96f60d6af8a2021-09-05T17:19:35ZengSciendoThe EuroBiotech Journal2564-615X2017-05-011220020110.24190/ISSN2564-615X/2017/02.18Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primersSysoliatin Eugeny Nikolaevich0Anisimova Natalia Vladimirovna1Babak Olga Gennadievna2Laboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusLaboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusLaboratory of Ecological Genetics and Biotechnology, Institute of Genetics and Cytology of NAS of Belarus, Minsk, BelarusWe examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.https://doi.org/10.24190/ISSN2564-615X/2017/02.18
collection DOAJ
language English
format Article
sources DOAJ
author Sysoliatin Eugeny Nikolaevich
Anisimova Natalia Vladimirovna
Babak Olga Gennadievna
spellingShingle Sysoliatin Eugeny Nikolaevich
Anisimova Natalia Vladimirovna
Babak Olga Gennadievna
Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
The EuroBiotech Journal
author_facet Sysoliatin Eugeny Nikolaevich
Anisimova Natalia Vladimirovna
Babak Olga Gennadievna
author_sort Sysoliatin Eugeny Nikolaevich
title Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
title_short Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
title_full Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
title_fullStr Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
title_full_unstemmed Genotyping Lupinus angustifolius cultivars with SRAP molecular markers and degenerate primers
title_sort genotyping lupinus angustifolius cultivars with srap molecular markers and degenerate primers
publisher Sciendo
series The EuroBiotech Journal
issn 2564-615X
publishDate 2017-05-01
description We examined 18 combinations of SRAP primers with resistance gene analog (RGA) and chitinase degenerate primers in order to determine their utility for genotyping L. angustifolius. Primer pairs ResAn51-f/Me8, p-loop/Em5, TM/Me8, Chit3-r/Em5 were the most effective for detection of genetic polymorphism of different narrow-leaved lupine varieties.
url https://doi.org/10.24190/ISSN2564-615X/2017/02.18
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AT anisimovanataliavladimirovna genotypinglupinusangustifoliuscultivarswithsrapmolecularmarkersanddegenerateprimers
AT babakolgagennadievna genotypinglupinusangustifoliuscultivarswithsrapmolecularmarkersanddegenerateprimers
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