| Summary: | <p>Abstract</p> <p>Background</p> <p>Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family <it>Togaviridae</it>. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia.</p> <p>Findings</p> <p>In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis.</p> <p>Conclusion</p> <p>INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine.</p>
|
|---|
