Nuclei isolation from surgically resected human hippocampus

Nuclei isolation from surgically resected human hippocampus

Summary: Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for...

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Bibliographic Details
Main Authors: Fatma Ayhan, Connor Douglas, Bradley C. Lega, Genevieve Konopka
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Cell separation/fractionation
Genomics
RNAseq
Microbiology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005505

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http://www.sciencedirect.com/science/article/pii/S2666166721005505

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