Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis

Cells have the ability to detect electric fields and respond to them with directed migratory movement. Investigations identified genes and proteins that play important roles in defining the migration efficiency. Nevertheless, the sensing and transduction mechanisms underlying directed cell migration...

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Main Authors: Isabella Guido, Douglas Diehl, Nora Aleida Olszok, Eberhard Bodenschatz, Xin Yi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500600/?tool=EBI
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spelling doaj-2ef581961e0f4e3895d7a2e11de4f9172020-11-25T03:41:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01159Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxisIsabella GuidoDouglas DiehlNora Aleida OlszokEberhard BodenschatzXin YiCells have the ability to detect electric fields and respond to them with directed migratory movement. Investigations identified genes and proteins that play important roles in defining the migration efficiency. Nevertheless, the sensing and transduction mechanisms underlying directed cell migration are still under discussion. We use Dictyostelium discoideum cells as model system for studying eukaryotic cell migration in DC electric fields. We have defined the temporal electric persistence to characterize the memory that cells have in a varying electric field. In addition to imposing a directional bias, we observed that the electric field influences the cellular kinematics by accelerating the movement of cells along their paths. Moreover, the study of vegetative and briefly starved cells provided insight into the electrical sensing of cells. We found evidence that conditioned medium of starved cells was able to trigger the electrical sensing of vegetative cells that would otherwise not orient themselves in the electric field. This observation may be explained by the presence of the conditioned medium factor (CMF), a protein secreted by the cells, when they begin to starve. The results of this study give new insights into understanding the mechanism that triggers the electrical sensing and transduces the external stimulus into directed cell migration. Finally, the observed increased mobility of cells over time in an electric field could offer a novel perspective towards wound healing assays.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500600/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Isabella Guido
Douglas Diehl
Nora Aleida Olszok
Eberhard Bodenschatz
Xin Yi
spellingShingle Isabella Guido
Douglas Diehl
Nora Aleida Olszok
Eberhard Bodenschatz
Xin Yi
Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
PLoS ONE
author_facet Isabella Guido
Douglas Diehl
Nora Aleida Olszok
Eberhard Bodenschatz
Xin Yi
author_sort Isabella Guido
title Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
title_short Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
title_full Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
title_fullStr Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
title_full_unstemmed Cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
title_sort cellular velocity, electrical persistence and sensing in developed and vegetative cells during electrotaxis
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2020-01-01
description Cells have the ability to detect electric fields and respond to them with directed migratory movement. Investigations identified genes and proteins that play important roles in defining the migration efficiency. Nevertheless, the sensing and transduction mechanisms underlying directed cell migration are still under discussion. We use Dictyostelium discoideum cells as model system for studying eukaryotic cell migration in DC electric fields. We have defined the temporal electric persistence to characterize the memory that cells have in a varying electric field. In addition to imposing a directional bias, we observed that the electric field influences the cellular kinematics by accelerating the movement of cells along their paths. Moreover, the study of vegetative and briefly starved cells provided insight into the electrical sensing of cells. We found evidence that conditioned medium of starved cells was able to trigger the electrical sensing of vegetative cells that would otherwise not orient themselves in the electric field. This observation may be explained by the presence of the conditioned medium factor (CMF), a protein secreted by the cells, when they begin to starve. The results of this study give new insights into understanding the mechanism that triggers the electrical sensing and transduces the external stimulus into directed cell migration. Finally, the observed increased mobility of cells over time in an electric field could offer a novel perspective towards wound healing assays.
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500600/?tool=EBI
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