The influence of using anticoagulants (EDTA and citrate acid 3.8%) toward the quantity of Platelet Rich Plasma (PRP)

• Main Author: Lilies Anggarwati Astuti
• Format: Article
• Language: English
• Published: Hasanuddin University 2016-06-01
• Series: Journal of Dentomaxillofacial Science
• Subjects: Anticoagulants, Quantity of platelet rich plasma
• Online Access: http://jdmfs.org/index.php/jdmfs/article/view/77
• ISSN: 2503-0817; 2503-0825

Platelet Rich Plasma (PRP) is a blood concentrate that has a thrombocytes concentration several time higher than normal concentration of thrombocytes in normal human blood. PRP is a promising alternative to surgery with a safe and natural healing. The standard protocol for PRP preparation must be determined to get the right quantity and quality of the matrix of fibrin, leukocytes, platelets and growth factors. It could not be separated from the number of PRP produced. The use of PRP in the success of periodontal treatment would not be separated from methods to obtain it. To detect the influence of using anticoagulants (EDTA and citrate acid 3.8%) toward the quantity of PRP. There are 41 subjects studied by taking 21 ml of venous blood in each of the seven tubes. Centrifugation performed twice with different speed, duration, use of anticoagulants then analyzed. This quantity between the two groups differed significantly between the PRP in EDTA group is higher 322.2 ml rather than citrate acid 3.8% group, then control group is higher 329.5 ml rather than citrate acid 3.8% group, while there is no difference between EDTA and control group. There is effect of the use of anticoagulants EDTA compared with citrate acid 3.8% in the quantity of PRP, and there was no effect using citrate acid 3.8% as anticoagulants in quantity of PRP.