Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci

• Main Authors: Thiago Galvão da Silva Paim, Vlademir Vicente Cantarelli, Pedro Alves d'Azevedo
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2014-06-01
• Series: Revista da Sociedade Brasileira de Medicina Tropical
• Subjects: Gram-positive cocci; Vitek 2 automated system
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000300377&lng=en&tlng=en

Introduction. The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positive human pathogens. The aim of this study was to evaluate the performance of Vitek 2 in identifying Gram-positive cocci and their antimicrobial susceptibilities. Methods. One hundred four isolates were analyzed to determine the accuracy of the automated system for identifying the bacteria and their susceptibility to oxacillin and vancomycin. Results. The system correctly identified 77.9% and 97.1% of the isolates at the species and genus levels, respectively. Additionally, 81.8% of the Vitek 2 results agreed with the known antimicrobial susceptibility profiles. Conclusion. Vitek 2 correctly identified the commonly isolated strains; however, the limitations of the method may lead to ambiguous findings.