Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk

With the present study aimed to evaluate the antagonistic action of Lactobacillus acidophilus front of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. In vitro tests were performed using Petri dishes with MRS agar and the milk fermented with Lactobacillus acidophilus also analyze...

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Main Authors: Neila Mello Cortez, Maria Carmela Kasnowski Holanda Duarte, Nayara Caldas de Macedo, Marco Antonio Sloboda Cortez, Robson Maia Franco
Format: Article
Language:English
Published: Instituto Federal de Educação, Ciência e Tecnologia do Amapá 2016-03-01
Series:Journal of Bioenergy and Food Science
Subjects:
Online Access:http://periodicos.ifap.edu.br/index.php/JBFS/article/view/79
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spelling doaj-2ecbbe1eebbc423eb51c35342b20ae402020-11-25T01:45:17ZengInstituto Federal de Educação, Ciência e Tecnologia do AmapáJournal of Bioenergy and Food Science2359-27102016-03-013111010.18067/jbfs.v3i1.79Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milkNeila Mello Cortez0Maria Carmela Kasnowski Holanda Duarte1Nayara Caldas de Macedo2Marco Antonio Sloboda Cortez3Robson Maia Franco4Universidade Federal de PernambucoUniversidade Federal FluminenseUniversidade Federal de PernambucoUniversidade Federal FluminenseUniversidade Federal FluminenseWith the present study aimed to evaluate the antagonistic action of Lactobacillus acidophilus front of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. In vitro tests were performed using Petri dishes with MRS agar and the milk fermented with Lactobacillus acidophilus also analyzed the chemistry and physical characteristics of the product during 35 days of storage under refrigeration. It has been observed in vitro formation of inhibition zones, ranging from 6 to 18 mm diameter, compared to the tested pathogens by lactic culture raises, indicating the possibility of producing organic acids, bacteriocins or other growth-inhibiting substances. In the fermented milk prepared, during storage, the probiotic micro-organism tested was able to inhibit the growth of E. coli O157: H7 and L. monocytogenes; and the log cycle reduction of S. aureus.http://periodicos.ifap.edu.br/index.php/JBFS/article/view/79Lactic acid bacteriaprobioticinhibitionL. monocytogenesE. coliS. aureus
collection DOAJ
language English
format Article
sources DOAJ
author Neila Mello Cortez
Maria Carmela Kasnowski Holanda Duarte
Nayara Caldas de Macedo
Marco Antonio Sloboda Cortez
Robson Maia Franco
spellingShingle Neila Mello Cortez
Maria Carmela Kasnowski Holanda Duarte
Nayara Caldas de Macedo
Marco Antonio Sloboda Cortez
Robson Maia Franco
Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
Journal of Bioenergy and Food Science
Lactic acid bacteria
probiotic
inhibition
L. monocytogenes
E. coli
S. aureus
author_facet Neila Mello Cortez
Maria Carmela Kasnowski Holanda Duarte
Nayara Caldas de Macedo
Marco Antonio Sloboda Cortez
Robson Maia Franco
author_sort Neila Mello Cortez
title Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
title_short Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
title_full Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
title_fullStr Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
title_full_unstemmed Lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
title_sort lactobacillus acidophilus antagonistic action against pathogenic strains inoculated in the fermented milk
publisher Instituto Federal de Educação, Ciência e Tecnologia do Amapá
series Journal of Bioenergy and Food Science
issn 2359-2710
publishDate 2016-03-01
description With the present study aimed to evaluate the antagonistic action of Lactobacillus acidophilus front of Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. In vitro tests were performed using Petri dishes with MRS agar and the milk fermented with Lactobacillus acidophilus also analyzed the chemistry and physical characteristics of the product during 35 days of storage under refrigeration. It has been observed in vitro formation of inhibition zones, ranging from 6 to 18 mm diameter, compared to the tested pathogens by lactic culture raises, indicating the possibility of producing organic acids, bacteriocins or other growth-inhibiting substances. In the fermented milk prepared, during storage, the probiotic micro-organism tested was able to inhibit the growth of E. coli O157: H7 and L. monocytogenes; and the log cycle reduction of S. aureus.
topic Lactic acid bacteria
probiotic
inhibition
L. monocytogenes
E. coli
S. aureus
url http://periodicos.ifap.edu.br/index.php/JBFS/article/view/79
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