Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein

• Main Authors: Susanti R., Retno D. Soejoedono, Gusti Ngurah I K Mahardika, Wayan T I Wibawan, Maggy T Suhartono
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7803

Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. This<br />research aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Java<br />based on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HA<br />genes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, and<br />sequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment<br />of nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.<br />The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns of<br />amino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all of<br />the viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.<br />Keywords: cleavage site, waterfowls, HPAI