Determination of protein in adipose tissue extracts
An accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265–275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-i...
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| Format: | Article |
| Language: | English |
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Elsevier
1976-09-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520417535 |
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doaj-2e7f9eb974f34777ae1ea8b424a080c02021-04-24T05:54:16ZengElsevierJournal of Lipid Research0022-22751976-09-01175542545Determination of protein in adipose tissue extractsH Tornqvist0P Belfrage1Department of Physiological Chemistry, Medical Faculty, University of Lund, Lund, SwedenDepartment of Physiological Chemistry, Medical Faculty, University of Lund, Lund, SwedenAn accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265–275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-increasing substance and the precipitate is extracted with an organic solvent. This interference caused by lipids, sulfhydryl reagents, and several other compounds is eliminated. The accuracy of the determination of dilute reference protein solutions (2–20 µg/ml) is demonstrated.http://www.sciencedirect.com/science/article/pii/S0022227520417535nonionic detergentTCA precipitationsolvent extraction |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
H Tornqvist P Belfrage |
| spellingShingle |
H Tornqvist P Belfrage Determination of protein in adipose tissue extracts Journal of Lipid Research nonionic detergent TCA precipitation solvent extraction |
| author_facet |
H Tornqvist P Belfrage |
| author_sort |
H Tornqvist |
| title |
Determination of protein in adipose tissue extracts |
| title_short |
Determination of protein in adipose tissue extracts |
| title_full |
Determination of protein in adipose tissue extracts |
| title_fullStr |
Determination of protein in adipose tissue extracts |
| title_full_unstemmed |
Determination of protein in adipose tissue extracts |
| title_sort |
determination of protein in adipose tissue extracts |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1976-09-01 |
| description |
An accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265–275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-increasing substance and the precipitate is extracted with an organic solvent. This interference caused by lipids, sulfhydryl reagents, and several other compounds is eliminated. The accuracy of the determination of dilute reference protein solutions (2–20 µg/ml) is demonstrated. |
| topic |
nonionic detergent TCA precipitation solvent extraction |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520417535 |
| work_keys_str_mv |
AT htornqvist determinationofproteininadiposetissueextracts AT pbelfrage determinationofproteininadiposetissueextracts |
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