| Summary: | Squamosa promoter-binding protein-like (SPL) is a major family of plant-specific transcription factor, which is involved in multiple biological processes, such as plant growth and development, hormone response, light response and stress response. Therefore, it has been profoundly significant to systematically analyze the SPL Transcription Factors family in <i>Brassica oleracea</i>. In this study, a total of 33 <i>BoSPLs</i> were identified in the <i>B. oleracea</i> genome, and they were further divided into six subgroups based on the phylogenetic tree constructed from the SPL proteins of <i>B. oleracea</i>, <i>B. rapa</i> and <i>Arabidopsis thaliana</i>. The expression profile of <i>BoSPLs</i> in different organs/tissues showed that a large number of <i>BoSPLs</i> were expressed in the callus, root, stem, leaf, bud, flower and silique. In addition, the expression levels of two <i>BoSPLs</i> (<i>BoSPL9b</i> and <i>BoSPL10b</i>) were up-regulated in chilling tolerance cabbage ‘CT-923’ at 6 h after chilling stress when compared with normal treatment (mock), while two <i>BoSPLs</i> (<i>BoSPL9b</i> and <i>BoSPL15a</i>) in chilling sensitive cabbage ‘CS-D9’, five <i>BoSPLs</i> (<i>BoSPL1</i>, <i>-9a, -9b, -10b, -11b</i>) in ‘CT-923’ and two <i>BoSPLs</i> (<i>BoSPL9b</i> and <i>BoSPL16a</i>) in ‘CS-D9’ were up-regulated after 24 h chilling stress, indicated that these genes may play an important role in the chilling-tolerance of cabbage. We analyzed the characteristics of <i>BoSPLs</i> and provided the basis for further functional research.
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