New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells

New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells

The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing c...

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Main Authors: Xiangjun He, Yin-Xiong Li, Bo Feng
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2018/9465028
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spelling doaj-2e27d4096fa14cea954b258f979373e52020-11-25T02:32:53ZengHindawi LimitedStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/94650289465028New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem CellsXiangjun He0Yin-Xiong Li1Bo Feng2Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong KongInstitute of Public Health, Guangdong Provincial Key Laboratory of Biocomputing, Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong KongThe groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.http://dx.doi.org/10.1155/2018/9465028
collection DOAJ
language English
format Article
sources DOAJ
author Xiangjun He
Yin-Xiong Li
Bo Feng
spellingShingle Xiangjun He
Yin-Xiong Li
Bo Feng
New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
Stem Cells International
author_facet Xiangjun He
Yin-Xiong Li
Bo Feng
author_sort Xiangjun He
title New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_short New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_full New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_fullStr New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_full_unstemmed New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_sort new turns for high efficiency knock-in of large dna in human pluripotent stem cells
publisher Hindawi Limited
series Stem Cells International
issn 1687-966X
1687-9678
publishDate 2018-01-01
description The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.
url http://dx.doi.org/10.1155/2018/9465028
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AT yinxiongli newturnsforhighefficiencyknockinoflargednainhumanpluripotentstemcells
AT bofeng newturnsforhighefficiencyknockinoflargednainhumanpluripotentstemcells
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