Effect of Magnetic Field on Adhesion of Muscle Cells to Culture Plate

• Main Authors: Shigehiro Hashimoto, Keiji Tachibana
• Format: Article
• Language: English
• Published: International Institute of Informatics and Cybernetics 2013-08-01
• Series: Journal of Systemics, Cybernetics and Informatics
• Subjects: Biomedical Engineering; Muscle Cells; Cell Culture; Magnetic Field; Adhesion; Orientation
• Online Access: http://www.iiisci.org/Journal/CV$/sci/pdfs/9B599QU.pdf

The effect of a magnetic field on adhesion of cultured muscle cells to the culture plate has been studied in vitro. An experimental system was manufactured to apply a magnetic field to muscle cell culture. The system consists of a couple of solenoid coils, a culture dish of 52 mm internal diameter, and an inverted phase-contrast microscope. The solenoid coil generates the alternating magnetic field of 13 mT of the effective value at a period of 0.01 s with the electric current of the rectangular pulses. C2C12 (Mouse myoblast cell line originated with cross-striated muscle of C3H mouse) cells were suspended in Dulbecco's Modified Eagle's Medium. The suspension was poured into the plastic dish placed on the stage of the microscope. The culture dish was exposed to the magnetic field between the solenoid coils at 29 degrees Celsius. For comparative study, a part of the suspension was poured into the same kind of dish without exposure to the magnetic field at 29 degrees Celsius. The number of cells, which adhered to the bottom of the culture dish, was traced according to the time (<130 min) during exposure to the alternating magnetic field. The experimental results show that adhesion is accelerated with alternating magnetic field of 13 mT.