Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase

Spectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.

Bibliographic Details
Main Author: I. Mulder
Format: Article
Language:English
Published: Elsevier 1972-07-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520393895
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spelling doaj-2e0eabb07af14f4bb820f047197901862021-04-24T05:51:34ZengElsevierJournal of Lipid Research0022-22751972-07-01134552554Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenaseI. Mulder0Laboratory of Veterinary Biochemistry, University of Utrecht, Utrecht, The NetherlandsSpectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.http://www.sciencedirect.com/science/article/pii/S0022227520393895acetoacetyl CoAthiolase
collection DOAJ
language English
format Article
sources DOAJ
author I. Mulder
spellingShingle I. Mulder
Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
Journal of Lipid Research
acetoacetyl CoA
thiolase
author_facet I. Mulder
author_sort I. Mulder
title Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
title_short Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
title_full Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
title_fullStr Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
title_full_unstemmed Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase
title_sort determination of acetyl coenzyme a. interference by a contaminant in malate dehydrogenase
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1972-07-01
description Spectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.
topic acetoacetyl CoA
thiolase
url http://www.sciencedirect.com/science/article/pii/S0022227520393895
work_keys_str_mv AT imulder determinationofacetylcoenzymeainterferencebyacontaminantinmalatedehydrogenase
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