Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome

Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with...

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Main Authors: L Karimzadeh, M Nabiuni, A Sheikholeslami, S Irian
Format: Article
Language:English
Published: SciELO 2012-01-01
Series:Journal of Venomous Animals and Toxins including Tropical Diseases
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006
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spelling doaj-2e0d8191c66842aeabb41a5c0e4065b52020-11-25T00:37:35ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992012-01-0118438439210.1590/S1678-91992012000400006Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndromeL KarimzadehM NabiuniA SheikholeslamiS IrianPolycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and livers were surgically removed to determine histomorphometrical changes. Testosterone and 17-β-estradiol were detected by chemiluminescence immunoassay. In order to detect serum CRP, ELISA kit was used in three groups of EV-induced PCOS, HBV-treated PCOS and control animals. Thickness of the theca layer, number of cysts and the level of serum CRP significantly decreased in HBV group in comparison with PCOS group. Moreover, corpus luteum, as a sign of ovulation, was observed in HBV-treated ovaries which were absent in PCOS group. Our results suggest that the beneficial effect of HBV may be mediated through its inhibitory effect on serum CRP levels.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006polycystic ovarian syndrome (PCOS)honeybee venom (HBV)C-reactive protein (CRP)estradiol valerate (EV)corpus luteumchemiluminescence immunoassay
collection DOAJ
language English
format Article
sources DOAJ
author L Karimzadeh
M Nabiuni
A Sheikholeslami
S Irian
spellingShingle L Karimzadeh
M Nabiuni
A Sheikholeslami
S Irian
Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
Journal of Venomous Animals and Toxins including Tropical Diseases
polycystic ovarian syndrome (PCOS)
honeybee venom (HBV)
C-reactive protein (CRP)
estradiol valerate (EV)
corpus luteum
chemiluminescence immunoassay
author_facet L Karimzadeh
M Nabiuni
A Sheikholeslami
S Irian
author_sort L Karimzadeh
title Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
title_short Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
title_full Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
title_fullStr Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
title_full_unstemmed Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
title_sort bee venom treatment reduced c-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
publisher SciELO
series Journal of Venomous Animals and Toxins including Tropical Diseases
issn 1678-9199
publishDate 2012-01-01
description Polycystic ovarian syndrome (PCOS) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. C-reactive protein (CRP), released by adipocytes, plays a key role in PCOS. Apis mellifera honeybee venom (HBV) contains a variety of biologically active components with various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent. To induce PCOS, 1 mg/100 g body weight estradiol valerate (EV) was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg HBV was administered SC for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with chloroform, and their ovaries and livers were surgically removed to determine histomorphometrical changes. Testosterone and 17-β-estradiol were detected by chemiluminescence immunoassay. In order to detect serum CRP, ELISA kit was used in three groups of EV-induced PCOS, HBV-treated PCOS and control animals. Thickness of the theca layer, number of cysts and the level of serum CRP significantly decreased in HBV group in comparison with PCOS group. Moreover, corpus luteum, as a sign of ovulation, was observed in HBV-treated ovaries which were absent in PCOS group. Our results suggest that the beneficial effect of HBV may be mediated through its inhibitory effect on serum CRP levels.
topic polycystic ovarian syndrome (PCOS)
honeybee venom (HBV)
C-reactive protein (CRP)
estradiol valerate (EV)
corpus luteum
chemiluminescence immunoassay
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400006
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