Extraction, Purification and Thermodynamic Characterization of Almond (Amygdalus communis) β-Galactosidase for the Preparation of Delactosed Milk

Buffer type, pH and ionic strength, as well as the fraction of polyvinylpyrrolidone were optimized for efficient extraction of β-galactosidase from almond seeds. The enzyme was purified up to electrophoretic homogeneity employing (NH4)2SO4 (15–60 %) fractionation, size exclusion and ion-exchange chr...

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Bibliographic Details
Main Authors: Melita Lobo, Farhath Khanum, Ajay Pal
Format: Article
Language:English
Published: University of Zagreb 2013-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/146868