Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis

Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis

TP-53 deficient cells of human leukaemia HL-60 die by massive apoptosis after treatment by high (50-100 nmol/l) doses of DNA damaging agent Idarubicin, regardless of the cell-cycle phase, in which they are affected. In contrary, after relatively low dose 10 nmol/l the cells die after cell-cycle arre...

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Main Authors: Martina Mareková, Jiřina Vávrová, Doris Vokurková
Format: Article
Language:English
Published: Karolinum Press 2000-01-01
Series:Acta Medica
Subjects:
HL-60
Idarubicin
Apoptosis
G2 cell-cycle arrest
Online Access:https://actamedica.lfhk.cuni.cz/43/2/0069/
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spelling doaj-2dce0a6542ff4fe3a617d2d7478168082020-11-25T00:44:54ZengKarolinum PressActa Medica1211-42861805-96942000-01-01432697310.14712/18059694.2019.116Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and ApoptosisMartina Mareková0Jiřina Vávrová1Doris Vokurková2Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicPurkyně Military Medical Academy, Hradec Králové, Institute of Radiobiology and Immunology, Hradec Králové, Czech RepublicUniversity Teaching Hospital in Hradec Králové, Institute of Clinical Immunology and Allergology, Hradec Králové, Czech RepublicTP-53 deficient cells of human leukaemia HL-60 die by massive apoptosis after treatment by high (50-100 nmol/l) doses of DNA damaging agent Idarubicin, regardless of the cell-cycle phase, in which they are affected. In contrary, after relatively low dose 10 nmol/l the cells die after cell-cycle arrest in G2 phase. The results show, that apoptosis induced by idarubicin could appear independently of the cell-cycle phase and that period in which apoptosis is observed is related to the dose of Idarubicin.https://actamedica.lfhk.cuni.cz/43/2/0069/HL-60IdarubicinApoptosisG2 cell-cycle arrest
collection DOAJ
language English
format Article
sources DOAJ
author Martina Mareková
Jiřina Vávrová
Doris Vokurková
spellingShingle Martina Mareková
Jiřina Vávrová
Doris Vokurková
Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
Acta Medica
HL-60
Idarubicin
Apoptosis
G2 cell-cycle arrest
author_facet Martina Mareková
Jiřina Vávrová
Doris Vokurková
author_sort Martina Mareková
title Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
title_short Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
title_full Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
title_fullStr Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
title_full_unstemmed Dose Dependent Biological Effects of Idarubicin in HL-60 Cells: Alterations of the Cell-Cycle and Apoptosis
title_sort dose dependent biological effects of idarubicin in hl-60 cells: alterations of the cell-cycle and apoptosis
publisher Karolinum Press
series Acta Medica
issn 1211-4286
1805-9694
publishDate 2000-01-01
description TP-53 deficient cells of human leukaemia HL-60 die by massive apoptosis after treatment by high (50-100 nmol/l) doses of DNA damaging agent Idarubicin, regardless of the cell-cycle phase, in which they are affected. In contrary, after relatively low dose 10 nmol/l the cells die after cell-cycle arrest in G2 phase. The results show, that apoptosis induced by idarubicin could appear independently of the cell-cycle phase and that period in which apoptosis is observed is related to the dose of Idarubicin.
topic HL-60
Idarubicin
Apoptosis
G2 cell-cycle arrest
url https://actamedica.lfhk.cuni.cz/43/2/0069/
work_keys_str_mv AT martinamarekova dosedependentbiologicaleffectsofidarubicininhl60cellsalterationsofthecellcycleandapoptosis
AT jirinavavrova dosedependentbiologicaleffectsofidarubicininhl60cellsalterationsofthecellcycleandapoptosis
AT dorisvokurkova dosedependentbiologicaleffectsofidarubicininhl60cellsalterationsofthecellcycleandapoptosis
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