Analysis of Single Nucleotide Variants in CRISPR-Cas9 Edited Zebrafish Exomes Shows No Evidence of Off-Target Inflation

Analysis of Single Nucleotide Variants in CRISPR-Cas9 Edited Zebrafish Exomes Shows No Evidence of Off-Target Inflation

Therapeutic applications of CRISPR-Cas9 gene editing have spurred innovation in Cas9 enzyme engineering and single guide RNA (sgRNA) design algorithms to minimize potential off-target events. While recent work in rodents outlines favorable conditions for specific editing and uses a trio design (moth...

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Bibliographic Details
Main Authors: Marie R. Mooney, Erica E. Davis, Nicholas Katsanis
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Genetics
Subjects:
CRISPR-Cas9
zebrafish
exome
de novo mutation
off-target effect
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00949/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00949/full

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