Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents

Introduction: Inflammation and oxidative stress are the main factors ascribed with interruption in the process of renal tissue impairment. The toxicity of different types of nitrosamine is well recognized in animals and humans. Administration of the smallest quantities of diethylnitrosamine or dimet...

Full description

Bibliographic Details
Main Authors: Deepika Singh, Deepak Chaudhary, Vikas Kumar, Amita Verma
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Toxicology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S221475002100055X
id doaj-2d97d44bf20b47db95dbd6fcab5b5a83
record_format Article
spelling doaj-2d97d44bf20b47db95dbd6fcab5b5a832021-03-27T04:27:44ZengElsevierToxicology Reports2214-75002021-01-018636645Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodentsDeepika Singh0Deepak Chaudhary1Vikas Kumar2Amita Verma3Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, 211007, India; Corresponding authors.Department of Pharmaceutical Sciences, Mohanlal Sukhadia University, Udaipur, Rajasthan, IndiaDepartment of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, 211007, IndiaDepartment of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad, Uttar Pradesh, 211007, India; Corresponding authors.Introduction: Inflammation and oxidative stress are the main factors ascribed with interruption in the process of renal tissue impairment. The toxicity of different types of nitrosamine is well recognized in animals and humans. Administration of the smallest quantities of diethylnitrosamine or dimethylnitrosamine either orally or parenterally results into renal damage. Therapeutic effects of phytofabricated silver nanoparticles of Carissa carandas aqueous extract has been scrutinised in current study for the assessment of renal cancer activity in animal model. Methodology: Phytofabricated silver nanoparticles were characterized by using different instrumentation. Nephroprotective activity of silver nanoparticles at different doses was evaluated against N-diethylnitrosamine (200 mg/kg b.w., intraperitoneal) in animal model. Serum and renal homogenate were taken to evaluate the renal toxicity markers, oxidative stress, and antioxidant parameter, proinflammatory cytokines and histopathological study. Result: Significant outcomes of silver nanoparticles in dose dependent manner down regulated the elevated serum marker, tumour marker enzymes and histopathology observation of repaired tissue assured the renal cancer activity in animals. In addition, profile of enzymatic and non-enzymatic antioxidant, proinflammatory cytokines and tumour promotion marker also favours the anticancer property of silver nanoparticles. Conclusion: The data of current study reveals silver nanoparticles ameliorates renal oxidative stress and carcinogenesis which was induced by N-diethylnitrosamine and accredited to antioxidant and anticancer activities of phytofabricated nanoparticles by biological approach.http://www.sciencedirect.com/science/article/pii/S221475002100055XCarissa carandas embedded silver nanoparticlesNF-κB pathwayRenal carcinoma
collection DOAJ
language English
format Article
sources DOAJ
author Deepika Singh
Deepak Chaudhary
Vikas Kumar
Amita Verma
spellingShingle Deepika Singh
Deepak Chaudhary
Vikas Kumar
Amita Verma
Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
Toxicology Reports
Carissa carandas embedded silver nanoparticles
NF-κB pathway
Renal carcinoma
author_facet Deepika Singh
Deepak Chaudhary
Vikas Kumar
Amita Verma
author_sort Deepika Singh
title Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
title_short Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
title_full Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
title_fullStr Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
title_full_unstemmed Amelioration of diethylnitrosamine (DEN) induced renal oxidative stress and inflammation by Carissa carandas embedded silver nanoparticles in rodents
title_sort amelioration of diethylnitrosamine (den) induced renal oxidative stress and inflammation by carissa carandas embedded silver nanoparticles in rodents
publisher Elsevier
series Toxicology Reports
issn 2214-7500
publishDate 2021-01-01
description Introduction: Inflammation and oxidative stress are the main factors ascribed with interruption in the process of renal tissue impairment. The toxicity of different types of nitrosamine is well recognized in animals and humans. Administration of the smallest quantities of diethylnitrosamine or dimethylnitrosamine either orally or parenterally results into renal damage. Therapeutic effects of phytofabricated silver nanoparticles of Carissa carandas aqueous extract has been scrutinised in current study for the assessment of renal cancer activity in animal model. Methodology: Phytofabricated silver nanoparticles were characterized by using different instrumentation. Nephroprotective activity of silver nanoparticles at different doses was evaluated against N-diethylnitrosamine (200 mg/kg b.w., intraperitoneal) in animal model. Serum and renal homogenate were taken to evaluate the renal toxicity markers, oxidative stress, and antioxidant parameter, proinflammatory cytokines and histopathological study. Result: Significant outcomes of silver nanoparticles in dose dependent manner down regulated the elevated serum marker, tumour marker enzymes and histopathology observation of repaired tissue assured the renal cancer activity in animals. In addition, profile of enzymatic and non-enzymatic antioxidant, proinflammatory cytokines and tumour promotion marker also favours the anticancer property of silver nanoparticles. Conclusion: The data of current study reveals silver nanoparticles ameliorates renal oxidative stress and carcinogenesis which was induced by N-diethylnitrosamine and accredited to antioxidant and anticancer activities of phytofabricated nanoparticles by biological approach.
topic Carissa carandas embedded silver nanoparticles
NF-κB pathway
Renal carcinoma
url http://www.sciencedirect.com/science/article/pii/S221475002100055X
work_keys_str_mv AT deepikasingh ameliorationofdiethylnitrosaminedeninducedrenaloxidativestressandinflammationbycarissacarandasembeddedsilvernanoparticlesinrodents
AT deepakchaudhary ameliorationofdiethylnitrosaminedeninducedrenaloxidativestressandinflammationbycarissacarandasembeddedsilvernanoparticlesinrodents
AT vikaskumar ameliorationofdiethylnitrosaminedeninducedrenaloxidativestressandinflammationbycarissacarandasembeddedsilvernanoparticlesinrodents
AT amitaverma ameliorationofdiethylnitrosaminedeninducedrenaloxidativestressandinflammationbycarissacarandasembeddedsilvernanoparticlesinrodents
_version_ 1724201359845097472