GeneXpert MTB/RIF Based Detection of Rifampicin Resistance and Common Mutations in rpoB Gene of Mycobacterium Tuberculosis in Tribal Population of District Anuppur, Madhya Pradesh, India
Introduction: Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) continues to be one of the most significant causes of death in the developing countries. Development of Multi Drug Resistance (MDR) and Extremely Drug Resistance (XDR) strains of MTB has been recognised as a major threat....
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2020-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/14035/45362_CE[Ra1]_F(SHU)_PF1(AB_SL)_PFA(SL)_PN(SL).pdf |
| Summary: | Introduction: Tuberculosis (TB) caused by Mycobacterium
tuberculosis (MTB) continues to be one of the most significant
causes of death in the developing countries. Development of
Multi Drug Resistance (MDR) and Extremely Drug Resistance
(XDR) strains of MTB has been recognised as a major threat.
Rapid diagnosis along with drug sensitivity analysis is the
prerequisite for effective treatment of TB, especially in rural and
remote location settings.
Aim: The goal of this study was to investigate the Rifampicin
Resistance (RR) using GeneXpert MTB/Rifampicin (RIF) in tribal
patients suffering from Pulmonary Tuberculosis (PTB) in District
Anuppur, Madhya Pradesh, India.
Materials and Methods: Sputum samples were obtained
from 413 patients with symptoms of PTB, who visited District
Hospital, Anuppur from April 2017- April 2018. Based on clinical
symptoms and chest X-ray, GeneXpert MTB/RIF assay was
performed for the confirmation of TB and detection of RR. The
data was analysed and expressed in percentage.
Results: Out of 413 samples, 104 (25.18%) were diagnosed
with PTB. Out of 104 TB positive samples, RR was detected
in 7(6.73%) samples. The most common mutations conferring
RR were located in the region of Probe B (71.42%), followed by
Probe C (14.28%) and Probe E (14.28%), while no mutations
were found in the region of Probe A and Probe D.
Conclusion: Possibly, this is the first report of RR and probe
mutational analysis from this tribal region of India. High
rate of mutation at Probe B locus may be the chief reason
for RR development. Gene sequencing may be carried for
understanding the higher rates of mutations at probe B locus. |
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| ISSN: | 2249-782X 0973-709X |