Enzymes Involved in Naproxen Degradation by Planococcus sp. S5

• Main Authors: Danuta Wojcieszyńska, Dorota Domaradzka, Katarzyna Hupert-Kocurek, Urszula Guzik
• Format: Article
• Language: English
• Published: Exeley Inc. 2016-06-01
• Series: Polish Journal of Microbiology
• Subjects: Planococcus; biodegradation; naproxen; enzymes induction; aromatic plant compounds
• Online Access: https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/2/pdf/10.5604_17331331.1204477.pdf

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a suf­ficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid or vanillic acid as growth substrates, the degradation of 21.5%, 71.71%, 14.75% and 8.16% of naproxen was observed respectively. It was shown that the activity of monooxygenase, hydroxyquinol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxyegnase in strain S5 was induced after growth of the strain with naproxen and 4-hydroxybenzoate. Moreover, in the presence of naproxen activity of gentisate 1,2-dioxygenase, enzyme engaged in 4-hydroxybenzoate metabolism, was completely inhibited. The obtained results suggest that monooxygenase and hydroxyquinol 1,2-dioxygenase are the main enzymes in naproxen degradation by Planococcus sp. S5.