A fluorescent-labeled microcystin-LR terbium cryptate
A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPL...
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Sociedade Brasileira de Química
2006-01-01
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| Series: | Journal of the Brazilian Chemical Society |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005 |
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doaj-2d5abebfdc2a4349ae52a025467659732020-11-25T01:24:03ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50531678-47902006-01-01172243250A fluorescent-labeled microcystin-LR terbium cryptateOliveira Eduardo J. A.Vila Nova Suzana P.Alves-Jr SeverinoSanta-Cruz PetrusMolica Renato J. R.Teixeira AntonioMalageño ElizabeteLima Filho José L.A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005terbium cryptatemicrocystin-LRcyanobacteria |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Oliveira Eduardo J. A. Vila Nova Suzana P. Alves-Jr Severino Santa-Cruz Petrus Molica Renato J. R. Teixeira Antonio Malageño Elizabete Lima Filho José L. |
| spellingShingle |
Oliveira Eduardo J. A. Vila Nova Suzana P. Alves-Jr Severino Santa-Cruz Petrus Molica Renato J. R. Teixeira Antonio Malageño Elizabete Lima Filho José L. A fluorescent-labeled microcystin-LR terbium cryptate Journal of the Brazilian Chemical Society terbium cryptate microcystin-LR cyanobacteria |
| author_facet |
Oliveira Eduardo J. A. Vila Nova Suzana P. Alves-Jr Severino Santa-Cruz Petrus Molica Renato J. R. Teixeira Antonio Malageño Elizabete Lima Filho José L. |
| author_sort |
Oliveira Eduardo J. A. |
| title |
A fluorescent-labeled microcystin-LR terbium cryptate |
| title_short |
A fluorescent-labeled microcystin-LR terbium cryptate |
| title_full |
A fluorescent-labeled microcystin-LR terbium cryptate |
| title_fullStr |
A fluorescent-labeled microcystin-LR terbium cryptate |
| title_full_unstemmed |
A fluorescent-labeled microcystin-LR terbium cryptate |
| title_sort |
fluorescent-labeled microcystin-lr terbium cryptate |
| publisher |
Sociedade Brasileira de Química |
| series |
Journal of the Brazilian Chemical Society |
| issn |
0103-5053 1678-4790 |
| publishDate |
2006-01-01 |
| description |
A new fluorescent labeled compound of microcystin-LR with terbium cryptate was obtained by initial conjugation of microcystins-LR with aminoethanethiol followed by the reaction with the ester group of terbium cryptate. The product formation was followed by high performance liquid chromatography (HPLC) at 238 nm and 310 nm. The presence of microcystin-LR in the labeled molecule was confirmed by enzyme linked immunosorbent assay ELISA and by protein reaction with bicinhonic acid. Luminescence spectra of cryptate and the conjugated molecule were carried through as well. |
| topic |
terbium cryptate microcystin-LR cyanobacteria |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200005 |
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