Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations]
Cationic modulation of the dominantly negative electrostatic structure of phospholipids plays an important role in bacterial response to changes in the environment. In addition to zwitterionic phosphatidylethanolamine, Gram-positive bacteria are also abundant in positively charged lysyl-phosphatidyl...
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doaj-2d48d4f8be394125af8ae90b9226c2c92020-11-25T03:50:53ZengF1000 Research LtdF1000Research2046-14022016-01-01510.12688/f1000research.7842.18441Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations]Metin Atila0Yu Luo1Department of Biochemistry, University of Saskatchewan, Saskatoon, CanadaDepartment of Biochemistry, University of Saskatchewan, Saskatoon, CanadaCationic modulation of the dominantly negative electrostatic structure of phospholipids plays an important role in bacterial response to changes in the environment. In addition to zwitterionic phosphatidylethanolamine, Gram-positive bacteria are also abundant in positively charged lysyl-phosphatidylglycerol. Increased amounts of both types of lipids render Gram-positive bacterial cells more resistant to cationic antibiotic peptides such as defensins. Lysyl and alanyl-phosphatidylglycerol as well as alanyl-cardiolipin have also been studied by mass spectroscopy. Phospholipids modified by other amino acids have been discovered by chemical analysis of the lipid lysate but have yet to be studied by mass spectroscopy. We exploited the high sensitivity of modern mass spectroscopy in searching for substructures in complex mixtures to establish a sensitive and thorough screen for aminoacylated phospholipids. The search for deprotonated aminoacyl anions in lipid extracted from Bacillus subtilis strain 168 yielded strong evidence as well as relative abundance of aminoacyl-phosphatidylglycerols, which serves as a crude measure of the specificity of aminoacyl-phosphatidylglycerol synthase MprF. No aminoacyl-cardiolipin was found. More importantly, the second most abundant species in this category is D-alanyl-phosphatidylglycerol, suggesting a possible role in the D-alanylation pathway of wall- and lipo-teichoic acids.http://f1000research.com/articles/5-121/v1Antimicrobials & Drug ResistanceBacterial Infections |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Metin Atila Yu Luo |
spellingShingle |
Metin Atila Yu Luo Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] F1000Research Antimicrobials & Drug Resistance Bacterial Infections |
author_facet |
Metin Atila Yu Luo |
author_sort |
Metin Atila |
title |
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
title_short |
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
title_full |
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
title_fullStr |
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
title_full_unstemmed |
Profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in Bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
title_sort |
profiling and tandem mass spectrometry analysis of aminoacylated phospholipids in bacillus subtilis [version 1; referees: 1 approved, 2 approved with reservations] |
publisher |
F1000 Research Ltd |
series |
F1000Research |
issn |
2046-1402 |
publishDate |
2016-01-01 |
description |
Cationic modulation of the dominantly negative electrostatic structure of phospholipids plays an important role in bacterial response to changes in the environment. In addition to zwitterionic phosphatidylethanolamine, Gram-positive bacteria are also abundant in positively charged lysyl-phosphatidylglycerol. Increased amounts of both types of lipids render Gram-positive bacterial cells more resistant to cationic antibiotic peptides such as defensins. Lysyl and alanyl-phosphatidylglycerol as well as alanyl-cardiolipin have also been studied by mass spectroscopy. Phospholipids modified by other amino acids have been discovered by chemical analysis of the lipid lysate but have yet to be studied by mass spectroscopy. We exploited the high sensitivity of modern mass spectroscopy in searching for substructures in complex mixtures to establish a sensitive and thorough screen for aminoacylated phospholipids. The search for deprotonated aminoacyl anions in lipid extracted from Bacillus subtilis strain 168 yielded strong evidence as well as relative abundance of aminoacyl-phosphatidylglycerols, which serves as a crude measure of the specificity of aminoacyl-phosphatidylglycerol synthase MprF. No aminoacyl-cardiolipin was found. More importantly, the second most abundant species in this category is D-alanyl-phosphatidylglycerol, suggesting a possible role in the D-alanylation pathway of wall- and lipo-teichoic acids. |
topic |
Antimicrobials & Drug Resistance Bacterial Infections |
url |
http://f1000research.com/articles/5-121/v1 |
work_keys_str_mv |
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