A Study of Bacterial and Fungal Isolates of Chronic Suppurative Otitis Media with their Antibiotic Susceptibility Pattern in Patients Attending a Tertiary Care Hospital

• Main Authors: R. Shamim, Anjana Gopi
• Format: Article
• Language: English
• Published: Journal of Pure and Applied Microbiology 2018-03-01
• Series: Journal of Pure and Applied Microbiology
• Subjects: chronic suppurative otitis media; bacterial isolates; fungal isolates; antibiotic susceptibility; antibiogram
• Online Access: https://microbiologyjournal.org/a-study-of-bacterial-and-fungal-isolates-of-chronic-suppurative-otitis-media-with-their-antibiotic-susceptibility-pattern-in-patients-attending-a-tertiary-care-hospital/

Chronic suppurative otitis media (CSOM) is a disease of multiple etiology and is known for its persistence and recurrence despite the treatment. The antibiotic resistance of the micro-organisms due to its inappropriate use make this potential dangerous condition difficult to treat. Hence the knowledge of the local epidemiology of the organism and its susceptibility to an antibiotic is essential to initiate an effective treatment. Objectives: 1. To study the bacterial and fungal isolates of Chronic Suppurative Otitis Media (CSOM); 2. To study the antibiotic susceptibility pattern of the isolates. The study was conducted among 100 cases with CSOM and 20 controls without CSOM attending ENT outpatient department at KIMS hospital for a duration of one year. The standard conventional method of isolation and identification was followed for isolating the organisms. The antibiogram for the isolates were determined using Kirby Bauer disk diffusion method. The data was analyzed using standard statistical package. Majority 30.0% among the cases with CSOM were in the age group of 21-30 yrs. 76.0% yielded single organism and 18.0% yielded multiple isolates. Among the cases with single isolates, Pseudomonas aeruginosa (44.7%) was the commonest isolate followed by Staphylococcus aureus (34.2%). Proteus mirabilis + Klebsiella oxytoca was the highest yielded (27.7%) isolate among the multiple isolates. Aspergillus species predominated the fungal isolates. 94.6% of the Pseudomonas aeruginosa isolates was susceptible to piperacillin followed by 67.6% to Gentamycin and 58.5% to Ciprofloxacin. 90.0% of Staphylococcus aureus isolates were sensitive to Gentamycin and Cotrimoxazole followed by Ceftriaxone (87.8%) and 66.6% were sensitive to penicillin. Among all the drugs, gentamycin was found be the effective drug for majority of the bacterial isolates. The study suggests that the common etiological agents for CSOM were Pseudomonas aeruginosa and Aspergillus niger. Gentamycin was the most sensitive drug for treatment of CSOM.