The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins

Background: Feeding intolerance (FI) is a common condition in premature infants that results in growth retardation and even necrotizing enterocolitis. The gut microbiome is linked to FI occurrence; however, the outcome after FI recovery is unclear.Methods: Fecal samples were collected from 11 pairs...

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Main Authors: Ying Li, Chunhong Jia, Xiaojun Lin, Lili Lin, Lizhen Li, Xi Fan, Xiaoxia Huang, Zhanyuan Xu, Huixin Wang, Fan Wu, Guosheng Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Pediatrics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fped.2021.648979/full
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spelling doaj-2d10890d41464550ab97c3b85ff25d442021-03-10T06:41:41ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602021-03-01910.3389/fped.2021.648979648979The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm TwinsYing Li0Ying Li1Ying Li2Chunhong Jia3Chunhong Jia4Xiaojun Lin5Lili Lin6Lizhen Li7Xi Fan8Xiaoxia Huang9Zhanyuan Xu10Huixin Wang11Fan Wu12Fan Wu13Guosheng Liu14Department of Neonatology, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaKey Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaKey Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Pediatrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaKey Laboratory for Major Obstetric Disease of Guangdong Province, Guangzhou, ChinaDepartment of Neonatology, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaBackground: Feeding intolerance (FI) is a common condition in premature infants that results in growth retardation and even necrotizing enterocolitis. The gut microbiome is linked to FI occurrence; however, the outcome after FI recovery is unclear.Methods: Fecal samples were collected from 11 pairs of premature twins/triplets for 16S rRNA gene sequencing. Initial fecal samples were collected shortly after admission, and then every other week until 7 weeks or discharge.Results: After FI recovery, there was no significant difference in the β-diversity of the intestinal flora between the FI group and the feeding tolerance (FT) group. By contrast, there was a significant difference in the β-diversity. Proteobacteria was the predominant phylum in the microbiome of the FI group, whereas Firmicutes was the predominant phylum in the microbiome of the FT group. The predominant bacteria with LDA >4 between the two groups at 13–15 days after birth, 19–28 days after birth, and at discharge were different, with the proportions of Bacillus, Clostridium butyricum, and Clostridium being highest in the FT group and Firmicutes, unidentified_Clostridiales, and Proteobacteria being highest in the FI group. Similarly, there were significant differences in the relative abundances of KEGG pathways, such as fatty acid metabolism, DNA repair and recombination proteins, energy metabolism, and amino acid metabolism, between the two groups (P < 0.01).Conclusions: There was a significant difference in diversity of the intestinal flora after feeding intolerance recovery. Feeding intolerance may disturb the succession of the intestinal bacterial community.https://www.frontiersin.org/articles/10.3389/fped.2021.648979/fullpremature infantstwinstripletsfeeding intolerancemicrobiome
collection DOAJ
language English
format Article
sources DOAJ
author Ying Li
Ying Li
Ying Li
Chunhong Jia
Chunhong Jia
Xiaojun Lin
Lili Lin
Lizhen Li
Xi Fan
Xiaoxia Huang
Zhanyuan Xu
Huixin Wang
Fan Wu
Fan Wu
Guosheng Liu
spellingShingle Ying Li
Ying Li
Ying Li
Chunhong Jia
Chunhong Jia
Xiaojun Lin
Lili Lin
Lizhen Li
Xi Fan
Xiaoxia Huang
Zhanyuan Xu
Huixin Wang
Fan Wu
Fan Wu
Guosheng Liu
The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
Frontiers in Pediatrics
premature infants
twins
triplets
feeding intolerance
microbiome
author_facet Ying Li
Ying Li
Ying Li
Chunhong Jia
Chunhong Jia
Xiaojun Lin
Lili Lin
Lizhen Li
Xi Fan
Xiaoxia Huang
Zhanyuan Xu
Huixin Wang
Fan Wu
Fan Wu
Guosheng Liu
author_sort Ying Li
title The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
title_short The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
title_full The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
title_fullStr The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
title_full_unstemmed The Diversity of the Intestinal Flora Disturbed After Feeding Intolerance Recovery in Preterm Twins
title_sort diversity of the intestinal flora disturbed after feeding intolerance recovery in preterm twins
publisher Frontiers Media S.A.
series Frontiers in Pediatrics
issn 2296-2360
publishDate 2021-03-01
description Background: Feeding intolerance (FI) is a common condition in premature infants that results in growth retardation and even necrotizing enterocolitis. The gut microbiome is linked to FI occurrence; however, the outcome after FI recovery is unclear.Methods: Fecal samples were collected from 11 pairs of premature twins/triplets for 16S rRNA gene sequencing. Initial fecal samples were collected shortly after admission, and then every other week until 7 weeks or discharge.Results: After FI recovery, there was no significant difference in the β-diversity of the intestinal flora between the FI group and the feeding tolerance (FT) group. By contrast, there was a significant difference in the β-diversity. Proteobacteria was the predominant phylum in the microbiome of the FI group, whereas Firmicutes was the predominant phylum in the microbiome of the FT group. The predominant bacteria with LDA >4 between the two groups at 13–15 days after birth, 19–28 days after birth, and at discharge were different, with the proportions of Bacillus, Clostridium butyricum, and Clostridium being highest in the FT group and Firmicutes, unidentified_Clostridiales, and Proteobacteria being highest in the FI group. Similarly, there were significant differences in the relative abundances of KEGG pathways, such as fatty acid metabolism, DNA repair and recombination proteins, energy metabolism, and amino acid metabolism, between the two groups (P < 0.01).Conclusions: There was a significant difference in diversity of the intestinal flora after feeding intolerance recovery. Feeding intolerance may disturb the succession of the intestinal bacterial community.
topic premature infants
twins
triplets
feeding intolerance
microbiome
url https://www.frontiersin.org/articles/10.3389/fped.2021.648979/full
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