Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid

Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid

Circulating tumor cells (CTCs) have attracted increasing attention as important biomarkers for clinical and biological applications. Several microfluidic approaches have been demonstrated to separate CTCs using immunoaffinity or size difference from other blood cells. This study demonstrates a sheat...

Full description

Bibliographic Details
Main Authors: Hyunjung Lim, Seung Min Back, Min Ho Hwang, Dae-Hee Lee, Hyuk Choi, Jeonghun Nam
Format: Article
Language:English
Published: MDPI AG 2019-07-01
Series:Micromachines
Subjects:
circulating tumor cell
white blood cell
sheathless
high-throughput
viscoelastic fluid
separation
Online Access:https://www.mdpi.com/2072-666X/10/7/462
id doaj-2ce471589ec8441b9a8ffc3e20e292ac
record_format Article
spelling doaj-2ce471589ec8441b9a8ffc3e20e292ac2020-11-25T01:52:32ZengMDPI AGMicromachines2072-666X2019-07-0110746210.3390/mi10070462mi10070462Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian FluidHyunjung Lim0Seung Min Back1Min Ho Hwang2Dae-Hee Lee3Hyuk Choi4Jeonghun Nam5Department of Medical Sciences, Graduate School of Medicine, Korea University, Seoul 02841, KoreaDepartment of Medical Sciences, Graduate School of Medicine, Korea University, Seoul 02841, KoreaDepartment of Medical Sciences, Graduate School of Medicine, Korea University, Seoul 02841, KoreaGraduate School of Medicine, Korea University College of Medicine, Seoul 02841, KoreaDepartment of Medical Sciences, Graduate School of Medicine, Korea University, Seoul 02841, KoreaDepartment of Laboratory Medicine, College of Medicine, Korea University Guro Hospital, Korea University, Seoul 08308, KoreaCirculating tumor cells (CTCs) have attracted increasing attention as important biomarkers for clinical and biological applications. Several microfluidic approaches have been demonstrated to separate CTCs using immunoaffinity or size difference from other blood cells. This study demonstrates a sheathless, high-throughput separation of CTCs from white blood cells (WBCs) using a viscoelastic fluid. To determine the fluid viscoelasticity and the flow rate for CTC separation, and to validate the device performance, flow characteristics of 6, 13, and 27 μm particles in viscoelastic fluids with various concentrations were estimated at different flow rates. Using 0.2% hyaluronic acid (HA) solution, MCF-7 (Michigan Cancer Foundation-7) cells mimicking CTCs in this study were successfully separated from WBCs at 500 μL/min with a separation efficiency of 94.8%. Small amounts of MCF-7 cells (~5.2%) were found at the center outlet due to the size overlap with WBCs.https://www.mdpi.com/2072-666X/10/7/462circulating tumor cellwhite blood cellsheathlesshigh-throughputviscoelastic fluidseparation
collection DOAJ
language English
format Article
sources DOAJ
author Hyunjung Lim
Seung Min Back
Min Ho Hwang
Dae-Hee Lee
Hyuk Choi
Jeonghun Nam
spellingShingle Hyunjung Lim
Seung Min Back
Min Ho Hwang
Dae-Hee Lee
Hyuk Choi
Jeonghun Nam
Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
Micromachines
circulating tumor cell
white blood cell
sheathless
high-throughput
viscoelastic fluid
separation
author_facet Hyunjung Lim
Seung Min Back
Min Ho Hwang
Dae-Hee Lee
Hyuk Choi
Jeonghun Nam
author_sort Hyunjung Lim
title Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
title_short Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
title_full Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
title_fullStr Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
title_full_unstemmed Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid
title_sort sheathless high-throughput circulating tumor cell separation using viscoelastic non-newtonian fluid
publisher MDPI AG
series Micromachines
issn 2072-666X
publishDate 2019-07-01
description Circulating tumor cells (CTCs) have attracted increasing attention as important biomarkers for clinical and biological applications. Several microfluidic approaches have been demonstrated to separate CTCs using immunoaffinity or size difference from other blood cells. This study demonstrates a sheathless, high-throughput separation of CTCs from white blood cells (WBCs) using a viscoelastic fluid. To determine the fluid viscoelasticity and the flow rate for CTC separation, and to validate the device performance, flow characteristics of 6, 13, and 27 μm particles in viscoelastic fluids with various concentrations were estimated at different flow rates. Using 0.2% hyaluronic acid (HA) solution, MCF-7 (Michigan Cancer Foundation-7) cells mimicking CTCs in this study were successfully separated from WBCs at 500 μL/min with a separation efficiency of 94.8%. Small amounts of MCF-7 cells (~5.2%) were found at the center outlet due to the size overlap with WBCs.
topic circulating tumor cell
white blood cell
sheathless
high-throughput
viscoelastic fluid
separation
url https://www.mdpi.com/2072-666X/10/7/462
work_keys_str_mv AT hyunjunglim sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
AT seungminback sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
AT minhohwang sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
AT daeheelee sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
AT hyukchoi sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
AT jeonghunnam sheathlesshighthroughputcirculatingtumorcellseparationusingviscoelasticnonnewtonianfluid
_version_ 1724994618610679808

Similar Items