Growth curve and development of the internal calli structure of Eucalyptus camaldulensis Dehn

• Main Authors: Evânia Galvão Mendonça, Luciano Vilela Paiva, Vanessa Cristina Stein, Marinês Ferreira Pires, Breno Régis Santos, Fabricio José Pereira
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2012-12-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: in vitro cultivation; organogenesis; anatomy; Astra blue; Safranin; forestry
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600012

The objective of this work was to elucidate the growth curve of Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect of the growth curve of the calli fresh matter was observed, with five different phases (lag, exponential, linear, deceleration and decline). In the lag phase, the highest growth percentage 87%, was observed, which reduced during the evaluation period to 17% in the linear phase. As for the anatomical analyses, cellular multiplications was observed during the lag and exponential phases and increase in cell size during the linear phase, promoting the calli volume growth and the establishment of the globular conformation.