Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy

Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy

In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We ev...

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Bibliographic Details
Main Authors: Márcio de Azevedo Figueiredo, Carlos Frederico Ceccon Lanes, Daniela Volcan Almeida, Luis Fernando Marins
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2007-01-01
Series:Genetics and Molecular Biology
Subjects:
transgenesis
genetically modified fish
microinjection
growth hormone cDNA
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100008

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