Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis

The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomia...

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Main Authors: Aoi Masuda, Sueli Fátima do Nascimento, Carmem Silvia Guerra, Gláucia da Silva Paranhos, Antonio Walter Ferreira
Format: Article
Language:English
Published: Universidade de São Paulo 1989-08-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
Antigens of Leishmania braziliensis braziliensis
Serodiagnosis of American cutaneous leishmaniasis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000400004&lng=en&tlng=en
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spelling doaj-2cac5e33c077490cb8b7aeecee4ca9b32020-11-24T22:27:59ZengUniversidade de São PauloRevista do Instituto de Medicina Tropical de São Paulo1678-99461989-08-0131422823410.1590/S0036-46651989000400004S0036-46651989000400004Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensisAoi Masuda0Sueli Fátima do Nascimento1Carmem Silvia Guerra2Gláucia da Silva Paranhos3Antonio Walter Ferreira4Universidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloUniversidade de São PauloThe antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000400004&lng=en&tlng=enAntigens of Leishmania braziliensis braziliensisSerodiagnosis of American cutaneous leishmaniasis
collection DOAJ
language English
format Article
sources DOAJ
author Aoi Masuda
Sueli Fátima do Nascimento
Carmem Silvia Guerra
Gláucia da Silva Paranhos
Antonio Walter Ferreira
spellingShingle Aoi Masuda
Sueli Fátima do Nascimento
Carmem Silvia Guerra
Gláucia da Silva Paranhos
Antonio Walter Ferreira
Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
Revista do Instituto de Medicina Tropical de São Paulo
Antigens of Leishmania braziliensis braziliensis
Serodiagnosis of American cutaneous leishmaniasis
author_facet Aoi Masuda
Sueli Fátima do Nascimento
Carmem Silvia Guerra
Gláucia da Silva Paranhos
Antonio Walter Ferreira
author_sort Aoi Masuda
title Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
title_short Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
title_full Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
title_fullStr Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
title_full_unstemmed Analysis of the specificity of human antibodies to antigens of Leishmania braziliensis braziliensis
title_sort analysis of the specificity of human antibodies to antigens of leishmania braziliensis braziliensis
publisher Universidade de São Paulo
series Revista do Instituto de Medicina Tropical de São Paulo
issn 1678-9946
publishDate 1989-08-01
description The antigenicity of promastigotes of Leishmania braziliensis braziliensis (L. b.braziliensis) treated with 1% sodium desoxycholate in 10 mM Tris-Hcl pH 8.2 was analysed by immunoblot using as probes sera from American cutaneous leishmaniasis (ACL), American visceral leishmaniasis (AVL), schistosomiasis, malaria and Chagas' disease. The ACL sera reacted constantly with a 60 kD band. No reactivity to this protein was observed with sera from the other diseases above mentioned indicating that the 60 kD protein may be used in serodiagnosis for ACL.
topic Antigens of Leishmania braziliensis braziliensis
Serodiagnosis of American cutaneous leishmaniasis
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651989000400004&lng=en&tlng=en
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