Trypanosoma cruzi: otimização da reação em cadeia da polimerase (PCR) no diagnóstico da doença de Chagas crônica e caracterização molecular de cepas isoladas de pacientes chagásicos crônicos no noroeste do Paraná Trypanosoma cruzi: use of an optimized PCR method in chronic Chagas disease diagnosis, and molecular characterization of strains isolated from chronic chagasic individuals living in northwest Paraná, Brazil

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