Comparative Fingerprint and Extraction Yield of Medicinal Herb Phenolics with Hepatoprotective Potential, as Determined by UV-Vis and FT-MIR Spectroscopy

• Main Authors: Simona ZAVOI, Florinela FETEA, Floricuta RANGA, Raluca M. POP, Anca BACIU, Carmen SOCACIU
• Format: Article
• Language: English
• Published: AcademicPres 2011-11-01
• Series: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
• Online Access: http://www.notulaebotanicae.ro/index.php/nbha/article/view/6278
• ISSN: 0255-965X; 1842-4309

The present study was aimed to compare the polyphenolic composition of six medicinal herbs, from wild flora of Romania. The plants investigated, Cynara scolimus (artichoke), Taraxacum officinalis (dandelion), Chelidonium majus (celandine), Hypericum perforatum (St. John’s wort), Silybum marianum (Mary thistle) and Lycopodium clavatum (Wolf’s claw) are known, to have hepatoprotective action. Using in parallel glycerol-water, ethanol-water and methanol, the solvent-dependence of the extract fingerprint and composition in bioactive molecules was studied by UV-Vis and Infrared (FT-MIR) spectrometry. The extraction yields, calculated as an extraction factor (EF) were superior in acidic methanol comparative to glycerin and ethanol, favorising the increase in phenolic acids against flavonoid derivatives . Based on the differences of polarity between the three solvents used, higher EF values were obtained for dandelion, artichoke, celandine and St. John wort, more rich in phenolic acids than flavonoids. Mary thistle and Wolf’s claw had lower concentrations of phenolics, but higher content of lignans and terpenoids. Based on the FT-MIR peaks from 8 regions, for each plant extract, has been determined the fingerprint region between 900 and 1500 cm-1and identified the specific functional groups. A good, significant correlation was found between the concentration of total phenolics calculated by UV-Vis spectrometry and FTIR methods, after calibration with gallic acid. The value of the MIR signal at 1743 cm-1 may be considered a good indicator of phenolics concentration in such extracts. Combined UV-Vis and FTIR spectroscopy are recommended as rapid and reliable tools to investigate the fingerprint and to predict the composition of medicinal plants or to evaluate the quality and authenticity of different standardized formulas.